ATP1A3 antibody (Intracellular)

Catalog No. ABIN7042962

This anti-ATP1A3 antibody is a Rabbit Polyclonal antibody detecting ATP1A3 in WB, IHC and IF. Suitable for Rat.

Quick Overview for ATP1A3 antibody (Intracellular) (ABIN7042962)

Target: ATP1A3 (ATPase, Na+/K+ Transporting, alpha 3 Polypeptide (ATP1A3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP1A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ATP1A3 Antibody

Binding Specificity: AA 3-15, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Alpha 3 Na+/K+ ATPase

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 11,13 amino acid residues identical

Characteristics: Anti-Alpha 3 Na+/K+ ATPase Antibody (ABIN7042962, ABIN7044641 and ABIN7044642) is a highly specific antibody directed against an epitope of the rat ATP1A3. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A3 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DKKDDKSSPKKSK, corresponding to amino acid residues 3-15 of rat ATP1A3 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: (ABIN7234848)

Blocking Peptide: (ABIN7234848)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ATP1A3

Target: ATP1A3 (ATPase, Na+/K+ Transporting, alpha 3 Polypeptide (ATP1A3))

Alternative Name: ATP1A3

Background: ATP1A3, Sodium/potassium ATPase catalytic subunit alpha-3, Sodium pump subunit alpha-3, Na+/K+-transporting ATPase subunit alpha-3,Alpha 3 Na+/K+ ATPase (ATP1A3) subunit is responsible for maintaining the electrochemical gradients of Na+ and K+ across the plasma membrane. A low intracellular Na+ concentration in eukaryotic cells is maintained by the Na+/K+ ATPase protein. Na+/K+ ATPase exports three sodium ions for every two potassium ions imported1-3.In most cells, Na+/K+ ATPase is distributed along the plasma membrane, whereas in epithelia the distribution is polarized in patterns specific to the type of epithelium. In the central nervous system (CNS), the α3 Na+/K+ ATPase isoform is expressed in neurons1,3.The establishment of an electrochemical gradient for Na+ and K+ across the plasma membrane is vital for cell function, it determines cell resting membrane potentials and supports the electrical activity of excitable cells. Autosomal dominant mutations of the human gene are related to a wide spectrum of clinical conditions, including several defined syndromes such as rapid-onset dystonia-parkinsonism, hyperactivity, epileptic seizures, optic atrophy, and sensorineural hearing loss2,3.

Alternative names: Alpha 3 Na+/K+ ATPase, ATP1A3, Sodium/potassium ATPase catalytic subunit alpha-3, Sodium pump subunit alpha-3, Na+/K+-transporting ATPase subunit alpha-3

Gene ID: 24213

NCBI Accession: NM_152296

UniProt: P06687

Pathways: Thyroid Hormone Synthesis, Proton Transport, Ribonucleoside Biosynthetic Process