BAI2 antibody (Extracellular, N-Term)

Catalog No. ABIN7886092

The Rabbit Polyclonal anti-BAI2 antibody is suitable to detect BAI2 in samples from Mouse. It has been validated for WB.

Quick Overview for BAI2 antibody (Extracellular, N-Term) (ABIN7886092)

Target: BAI2 (Brain-Specific Angiogenesis Inhibitor 2 (BAI2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAI2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BAI2 Antibody

Binding Specificity: AA 277-290, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to BAI2

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-BAI2 (extracellular) Antibody (ABIN7042973, ABIN7043945 and ABIN7043946) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize BAI2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EPKVKTQWPRSADE, corresponding to amino acid residues 277-290 of mouse BAI2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234884)

Blocking Peptide: (ABIN7234884)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for BAI2

Target: BAI2 (Brain-Specific Angiogenesis Inhibitor 2 (BAI2))

Alternative Name: ADGRB2

Background:

Synonyms: Brain-specific angiogenesis inhibitor 2, Adhesion G protein-coupled receptor B2, ADGRB2

Description: Brain-specific angiogenesis inhibitor 2 (BAI2) is a member of the adhesion G-protein coupled receptor (GPCR) family. BAI2, and other members of its family, commonly have a long N-terminal extracellular region (ECR) containing a GPCR proteolysis site (GPS) adjacent to the seven-transmembrane (7TM) domain. The long N-terminus contains common structural domains, including thrombospondin type-I repeats (TSRs), epidermal growth factor (EGF)-like, leucine-rich repeats, lectin-like, immunoglobulin (Ig), and cadherins. BAI2 is composed of 521 amino acids and is mainly expressed in neurons.Although BAI2 is a GPCR, there are currently no reports showing evidence of any physiological ligands. Instead, BAI2 is activated by cleavage and dissociation of the ECR from its 7TM fragment, and functions as a GPCR. BAI2 is glycosylated and cleaved at the GPS by furin in the ER. The cleaved N-terminal ECR associates with the C-terminal 7TM fragment and they are translocated to the cell surface. The ECR associated with the 7TM fragment interacts with other membrane proteins or matrix proteins through the TSRs and dissociates from the 7TM fragment. The 7TM fragment which has released the associated ECR changes its conformation into the active state and activates the G protein-coupled signaling1.BAI2 is involved in the early stages of neovascularization of cerebral cortex after ischemia. Decreased levels of BAI2 induce increased VEGF expression mediated by GA-binding protein gamma (GABPγ) in cerebral ischemia and under normal conditions2.

Gene ID: 230775

NCBI Accession: NM_001703

UniProt: Q8CGM1