GPR77 antibody (Extracellular)

Catalog No. ABIN7042975

Product Details anti-GPR77 Antibody

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Binding Specificity: AA 179-193, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR77 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal antibody to C5aR2 (extracellular).

Specificity: Extracellular, 2nd loop.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Will not recognize Human C5aR2.

Predicted Reactivity: Mouse - 14 out of 15 amino acid residues identical, Rat - 13 out of 15 amino acid residues identical Will not recognize Human C5aR2

Characteristics: A rabbit polyclonal antibody to C5aR2 (extracellular).

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RRLLQEHYPPRLV(S)G, corresponding to amino acid residues 179 - 193 of mouse C5aR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-AR034

Blocking Peptide: BLP-AR034

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR77

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Alternative Name: C5AR2 (GPR77 Products)

Background: Complement Component 5a Receptor 2, C5L2, C5a Anaphylatoxin Chemotactic Receptor 2, G Protein-Coupled Receptor 77, GPR77,The complement system is a vital component of the innate immune system that play an important role in the immune responses to infectious organisms and tissue damages 1. The activation of the complement system is carried out through a proteolytic enzymes cascade generating various peptides and fragments 2.One of the most important component of the complement system is a protein named complement protein C5 3. Upon proteolytic cleavage of C5 two peptides are generated, C5a and C5b 4. C5a is known to be the most potent chemotactic factor in cells and responsible for the directed migration of cells involved in the immune response to infectious organisms and tissue damages 5. There are two different C5a receptors, C5aR1 (C5aR, CD88) and C5aR2 (C5L2, GPR77). C5aR1 is a member of the G-protein coupled receptors (GPCRs), and known to mediate the vast majority of C5a biological activity 6. In contrast C5aR2 does not exhibit any functional coupling to G proteins and it exact mechanism of action remains controversial 7. Expression of C5aR2 can be found in various tissues such as, bone marrow, spleen, lung and in immune cells such as myeloid cells and T cells 3.

Alternative names: Complement Component 5a Receptor 2, C5L2, C5a Anaphylatoxin Chemotactic Receptor 2, G Protein-Coupled Receptor 77, GPR77

Gene ID: 319430

NCBI Accession: NM_018485

UniProt: Q8BW93