Stargazin antibody (Intracellular)

Catalog No. ABIN7042989

The Rabbit Polyclonal anti-Stargazin antibody (ABIN7042989) specifically detects Stargazin in WB, IF and IC. The antibody is reactive with Mouse samples.

Quick Overview for Stargazin antibody (Intracellular) (ABIN7042989)

Target: Stargazin (CACNG2) (Calcium Channel, Voltage-Dependent, gamma Subunit 2 (CACNG2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Stargazin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-Stargazin Antibody

Binding Specificity: AA 213-228, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Stargazin (CaV?2 Subunit)

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-Stargazin Antibody is directed against an epitope of rat CaVγ2. Anti-Stargazin Antibody (ABIN7042989 and ABIN7043965) can be used in western blot analysis. It has been designed to recognize CaVγ2 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RATDYLQASAITRIPS, corresponding to amino acid residues 213-228 of mouse CaVgamma2 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234938)

Blocking Peptide: (ABIN7234938)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Stargazin

Target: Stargazin (CACNG2) (Calcium Channel, Voltage-Dependent, gamma Subunit 2 (CACNG2))

Alternative Name: CACNG2

Background: CaVγ2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler,The γ subunit protein is an auxiliary subunit of the CaV (voltage-dependent calcium) channel multimer complex. γ1 was first identified in skeletal muscle as a part of the proteins complex purified as the DHP receptor. Eight γ subunit isoforms were identified in mammalian genomes and they share some sequence as well as structure homology.1 γ subunit contains four transmembrane helices, with intracellular N- and C- termini. Usually their association with the pore-forming α1 subunit results in reduced current amplitude.CaVγ2 is also known as Stargazin, and interacts and regulates the activity and trafficking of AMPA glutamate receptors.2

Alternative names: Stargazin, CaVgamma2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler

Gene ID: 12300

NCBI Accession: NM_001379051

UniProt: O88602

Pathways: Skeletal Muscle Fiber Development