CACNG8 antibody (Intracellular)

Catalog No. ABIN7042996

This Rabbit Polyclonal antibody specifically detects CACNG8 in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for CACNG8 antibody (Intracellular) (ABIN7042996)

Target: CACNG8 (Calcium Channel, Voltage-Dependent, gamma Subunit 8 (CACNG8))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNG8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CACNG8 Antibody

Binding Specificity: AA 2-17, Intracellular

Purpose: A Rabbit Polyclonal Antibody to CACNG8 (CaV?8) Subunit

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat,mouse,human - 15,16 amino acid residues identical

Characteristics: Anti-CACNG8 Antibody is directed against an epitope of rat TARP gamma-8 (CaVγ8, CACNG8). Anti-CACNG8 Antibody (ABIN7042996, ABIN7044073 and ABIN7044074) can be used in western blot and immunohistochemistry applications. It has been designed to recognize TARP gamma-8 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ESLKRWNEERGLW(S)EK, corresponding to amino acid residues 2-17 of rat CACNG8 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7234961)

Blocking Peptide: (ABIN7234961)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CACNG8

Target: CACNG8 (Calcium Channel, Voltage-Dependent, gamma Subunit 8 (CACNG8))

Alternative Name: CACNG8

Background: CaVγ8, Voltage-dependent calcium channel gamma-8 subunit, TARP gamma-8, Transmembrane AMPAR regulatory protein gamma-8,Transmembrane AMPA receptor regulatory proteins (TARPs) serve as auxiliary subunits of AMPA receptors that regulate functional aspects of these receptors such as: fast excitatory synaptic transmission, surface trafficking, enhancing synaptic clustering and increasing glutamate affinity. Generally, TARPs are responsible for regulating expression, channel properties and localization of AMPA receptors in the brain1.TARPs are non-pore-forming integral membrane proteins with four transmembrane domains that are widely expressed in the CNS. The TARP family is divided in Type I and type II subfamily. Type I TARPs comprise four calcium channel γ subunits: γ2, γ3, γ4, and γ-8, also known as Cacng2, 3, 4 and 8, respectively2,3.γ-8 TARP predominantly expressed in the telencephalon with abundant distribution on hippocampal excitatory synapses and extrasynaptic membranes. It is expressed in low levels in newborn and neonatal brain and can be found in higher levels in adult brain4. γ-8 plays an important role in increasing the number of synaptic and extrasynaptic AMPA receptors on dendrites and spines5. The C terminus of γ-8 contains 3 unique stretches of amino acids that are not found in any of the other TARPs. In addition, it contains PDZ-binding motifs2. In heterologous cells, γ-8 prolong the current rise time in response to small amounts of glutamate and slow GluA1 receptor desensitization and deactivation3.Several studies have shown that overexpression of TARP γ-8 increases the number of AMPARs in the plasma membrane. In γ-8 knockout mice, AMPA receptor expression is severely reduced from the synaptic and extrasynaptic membranes of dendrites and does not progress through the secretory pathway5,6.

Alternative names: CACNG8, CaVgamma8, Voltage-dependent calcium channel gamma-8 subunit, TARP gamma-8, Transmembrane AMPAR regulatory protein gamma-8

Gene ID: 140729

NCBI Accession: NM_031895

UniProt: Q8VHW5