CACNA1B antibody (Intracellular)

Catalog No. ABIN7043005

This Rabbit Polyclonal antibody specifically detects CACNA1B in WB, IHC, IF, IP and IC. It exhibits reactivity toward Rat.

Quick Overview for CACNA1B antibody (Intracellular) (ABIN7043005)

Target: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACNA1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-CACNA1B Antibody

Binding Specificity: AA 851-867, Intracellular

Purpose: A Rabbit Polyclonal Antibody to CACNA1B (CaV2.2) Channel

Specificity: Intracellular loop between domains II and III

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: rabbit - 11,Mouse - 15,17 amino acid residues identical, human 11

Characteristics: Anti-CACNA1B (CaV2.2) Antibody (ABIN7043005, ABIN7043951 and ABIN7043952) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV2.2 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 of rat CACNA1B 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC

Negative Control: (ABIN7234980)

Blocking Peptide: (ABIN7234980)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CACNA1B

Target: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Alternative Name: CACNA1B

Background: Voltage-dependent N-type calcium channel subunit α1B, Brain calcium channel III, BIII,Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1The voltage-dependent Ca2+ channels are composed of several subunits, α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.The CaV2.2 (formally named α1B) composes the α1 poreforming subunit for the N-type Ca2+ channel family. They are involved in neurotransmitter release from central neurons, including glutamate, γ-aminobutyric acid, acetylcholine, dopamine and noradrenaline.2The CaV2.2 is expressed preferentially in the central nervous system, where along with CaV2.1, it is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,3The CaV2.2 channel is negatively regulated by many different GPCRs. There are two ways that this is done: either by directly binding Gβγ to the channel or by an indirect mechanism involving second messenger and channel phosphorylation.4 ω-Conotoxin GVIA (#C-300) is a specific blocker of CaV2.2 Ca2+ channels. It specifically blocks N-type CaV channels by binding to the CaV2.2 α1 subunit (α1B) and its action is only partially reversible.5,6

Alternative names: CACNA1B (CaV2.2), Voltage-dependent N-type calcium channel subunit alpha1B, Brain calcium channel III, BIII

Gene ID: 257648

NCBI Accession: NM_000718

UniProt: Q02294