CNR2 antibody (AA 228-242)

Catalog No. ABIN7043025

Product Details anti-CNR2 Antibody

Target: CNR2 (Cannabinoid Receptor 2 (CNR2))

Binding Specificity: AA 228-242

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2

Specificity: 3rd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical Human - 14 out of 15 amino acid residues identical

Characteristics: Anti-Cannabinoid Receptor 2 Antibody (ABIN7043025, ABIN7044157 and ABIN7044158) is directed against an intracellular epitope of the rat CB2 receptor. Anti-Cannabinoid Receptor 2 Antibody (ABIN7043025, ABIN7044157 and ABIN7044158) can be used in western blot and immunohistochemistry applications, and recognizes CB2 in rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DRQVPGIARMRLDVR, corresponding to amino acid residues 228-242 of rat CB2 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100-1:400

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: (ABIN7235032)

Blocking Peptide: (ABIN7235032)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CNR2

Target: CNR2 (Cannabinoid Receptor 2 (CNR2))

Alternative Name: CNR2 (CNR2 Products)

Background: CB2, CNR2,Cannabinoids have been used as pain relievers in Eastern medicine for many years.1 To date, two specific cannabinoid receptors have been identified: cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2).2 The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution.2 Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein.2,3The CB2 receptor is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas.1,2,4 CB2 expression in the brain is still much less characterized than that of CB1. Recently, it was demonstrated that CB2 is expressed in the brain and might have a role in controlling fundamental processes such as proliferation and survival of neural cells.5,6Overexpression of CB2 was reported in several cancers such as prostate, glioma and acute myeloid leukemias.6 In human astrocytoma a direct relationship between CB2 expression and tumor malignancy was demonstrated. Activation of CB2 in vivo by its agonist JWH-133, completely blocked cell growth. In C6 glioma, it was shown that activation of the CB2 by JWH-133 resulted in the internalization of only the CB2 and not CB1 leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma.7

Alternative names: Cannabinoid Receptor 2, CB2, CNR2

Gene ID: 57302

NCBI Accession: NM_001841

UniProt: Q9QZN9