CATSPER1 antibody (3rd Extracellular Loop)

Catalog No. ABIN7886204

The Rabbit Polyclonal anti-CATSPER1 antibody is suitable to detect CATSPER1 in samples from Mouse. It has been validated for WB.

Quick Overview for CATSPER1 antibody (3rd Extracellular Loop) (ABIN7886204)

Target: CATSPER1 (Cation Channel, Sperm Associated 1 (CATSPER1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CATSPER1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CATSPER1 Antibody

Binding Specificity: 3rd Extracellular Loop, AA 508-521

Purpose: A Rabbit Polyclonal Antibody to CatSper1 Channel

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat,human - 12,14 amino acid residues identical

Characteristics: Anti-CatSper1 (extracellular) Antibody (ABIN7043027 and ABIN7044102) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting CatSper1 in living cells. It has been designed to recognize CatSper1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RALFQDSDPKRFQN, corresponding to amino acid residues 508-521 of mouse CatSper1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235036)

Blocking Peptide: (ABIN7235036)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CATSPER1

Target: CATSPER1 (Cation Channel, Sperm Associated 1 (CATSPER1))

Alternative Name: CATSPER1

Background:

Synonyms: Cation channel sperm-associated protein 1

Description: Capacitation is the final stage of spermatozoon maturation which eventually enables it to bind the zona pellucida (ZP) - a thick layer of glycoproteins surrounding the oocyte1-3. It occurs in the fallopian tubes of the female reproductive tract and is preceded by spermatogenesis (testes) and epididymal maturation (epididymis)3,4. Capacitation is accompanied by drastic morphological and functional alterations, such as membrane hyperpolarization, changes in membrane lipid composition, intracellular alkalinization, increased level of protein tyrosine phosphorylation by cAMP-dependent PKA, and elevated intracellular concentrations of Ca2+ ([Ca2+]i) and pH i1,2, which render the sperm chemotaxis-responsive and hyperactive6. Increased flagellar bend, triggered by Ca2+ signaling, produces asymmetrical, whiplash-like motility pattern that provides the sperm with significantly stronger swimming thrust1-3. Hyperactivity, together with the eruption of hydrolytic enzymes from the sperm's acrosome vesicle facilitate the disruption of the ZP matrix and make way for the sperm to enter the egg cytoplasm1-7. CatSper (cation channel of spermatozoa) is a sperm-specific Ca2+ channel located explicitly in the spermatozoon flagellum2. It mediates flagellar hyperactivity7, and is chemotactically responsive to progesterone8. CatSper is composed of 4 pore-forming subunits (CatSper1-4) with 6 transmembrane (TM) domains each, and of 3 auxiliary subunits: CatSperβ (2 TM domains), CatSperγ and CatSperδ (1 TM domain each)1, 2. While all four CatSper proteins are necessary for the channel's function (and hence male fertility)9, each is synthesized independently. Trafficking to the flagellum requires the cooperation of all subunits1,3.CatSper1, the first identified subunit of the channel, is highly homologous to CaV channels, a voltage sensor domain (S1-S4) containing enough positively charged residues to confer weak voltage sensitivity to the channel2. Furthermore, its histidine-rich, intracellular amino terminus likely serves as the pH sensor necessary to induce hyperactivation (by permitting an influx of Ca2+)3. Indeed, intracellular alkaline pH (7.9-8.5) was shown to efficiently hyper-activate CatSper channels6.

Gene ID: 225865

NCBI Accession: NM_053054

UniProt: Q91ZR5