CATSPER4 antibody (1st Extracellular Loop)

Catalog No. ABIN7886205

The Rabbit Polyclonal anti-CATSPER4 antibody (ABIN7886205) specifically detects CATSPER4 in WB, IF and IC. The antibody is reactive with Mouse samples.

Quick Overview for CATSPER4 antibody (1st Extracellular Loop) (ABIN7886205)

Target: CATSPER4 (Cation Channel, Sperm Associated 4 (CATSPER4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CATSPER4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-CATSPER4 Antibody

Binding Specificity: 1st Extracellular Loop, AA 90-105

Purpose: A Rabbit Polyclonal Antibody to CatSper4 Channel

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat - identical, human -15,16 amino acid residues identical

Characteristics: Anti-CatSper4 (extracellular) Antibody is directed against an epitope of mouse CatSper4. Anti-CatSper4 (extracellular) Antibody (ABIN7043028, ABIN7044103 and ABIN7044104) can be used in western blot and immunocytochemistry applications. It has been designed to recognize CatSper4 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)NSYLGQKHYELFSTID, corresponding to amino acid residues 90-105 of mouse CatSper4

Isotype: IgG

Application Details

Application Notes:

WB: 1:1000

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: ICC

Negative Control: (ABIN7235039)

Blocking Peptide: (ABIN7235039)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CATSPER4

Target: CATSPER4 (Cation Channel, Sperm Associated 4 (CATSPER4))

Alternative Name: CATSPER4

Background:

Synonyms: Cation channel sperm-associated protein 4

Description: During mammalian fertilization, Ca2+ signaling plays a central role in sperm capacitation and motility to the fusion between sperm and egg1. Several Ca2+-permeable ion channel proteins have been characterized in mammalian sperm. However, only the four mammalian CatSper members (CatSpers 1-4) are restrictively expressed in the testis and are clearly shown to be required for male fertility2.CatSper channels are named after the first putative cation channel of sperm3. CatSper4 contains a single ion transport domain comprised of 6 transmembrane spanning regions, where the fourth transmembrane region resembles a voltage sensor and a pore forming region lies between transmembrane regions 5 and 6. The pore contains the consensus sequence TxDxW indicative of a probable Ca2+ selective channel4. The four CatSper proteins are primarily localized to the plasma membrane of the distal portion of the sperm tail and presumably form a heterotetrameric, pH - and voltage-dependent Ca2+-permeable channel 5. CatSper 4 is highly expressed in testis and expressed at low levels in placenta and lung6.Male mice deficient in any of the four CatSper are completely sterile but exhibit no other apparent abnormality. The major phenotypes of the null sperm are a lack of hyperactivated motility, a gradual loss of motility after isolation, and an inability to penetrate zona-intact eggs7.

Gene ID: 329954

NCBI Accession: NM_198137

UniProt: Q8BVN3