ADGRE5 (AA 499-512), (Extracellular) antibody

Catalog No. ABIN7043030

The Rabbit Polyclonal anti- antibody has been validated for WB and FACS. It is suitable to detect in samples from Human.

Quick Overview for ADGRE5 (AA 499-512), (Extracellular) antibody (ABIN7043030)

Target: ADGRE5

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details

Binding Specificity: AA 499-512, Extracellular

Purpose: A Rabbit Polyclonal Antibody to CD97/ADGRE5

Specificity: Extracellular, N-terminus

Cross-Reactivity (Details): The antibody will not recognize the protein from rat or mouse samples.

Predicted Reactivity: Human only

Characteristics: Anti-Human CD97/ADGRE5 (extracellular) Antibody (ABIN7043030, ABIN7044271 and ABIN7044272) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CD97 from human samples only. The antibody will not recognize the protein from rat or mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KSDSDRGGHWATEG, corresponding to amino acid residues 499-512 of human CD97/ADGRE5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details

Target: ADGRE5

Background: Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97, Seven-transmembrane heterodimeric receptor associated with inflammation,CD97, encoded by the adhesion G protein-coupled receptor 5 (ADGRE5) gene, is a member of the epidermal growth factor (EGF) seven transmembrane receptor family, belonging to the adhesion family of G protein-coupled receptors (GPCRs)1. CD97 is widely expressed on the surface of lymphoid cells, macrophages, smooth muscle cells and several types of tumor cells and acts through binding of decay accelerating factor (CD55) and dermatan sulfate2.In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation3. Antibodies against CD97 have been demonstrated to diminish various inflammatory disorders by depleting granulocytes and the interaction between CD97 and its ligand CD55 regulates T-cell activation and increases proliferation and cytokine production4.Changes in the expression of CD97 have been described in auto-inflammatory diseases, such as rheumatoid arthritis and multiple sclerosis. Outside the immune system, CD97 is likely involved in cell-cell interactions and ectopic CD97 expression leading to enhanced cell-cell aggregation5.

Alternative names: Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97, Seven-transmembrane heterodimeric receptor associated with inflammation

Gene ID: 976

NCBI Accession: NM_078481

UniProt: P48960