CDNF antibody (Mature)

Catalog No. ABIN7043031

Product Details anti-CDNF Antibody

Target: CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))

Binding Specificity: AA 129-141, Mature

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDNF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Cerebral Dopamine Neurotrophic Factor

Specificity: Mature CDNF protein

Cross-Reactivity: Human, Mouse

Cross-Reactivity (Details): Not recommended for rat samples.

Predicted Reactivity: Mouse - 10,13 amino acid residues identical

Characteristics: Anti-CDNF Antibody is directed against an epitope of human CDNF. Anti-CDNF Antibody (ABIN7043031, ABIN7044785 and ABIN7044786) can be used in western blot and immunohistochemistry applications. It has been designed to recognize CDNF from mouse and human samples. The antibody will not recognize CDNF from rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EKTLDLASVDLRK, corresponding to amino acid residues 129-141 of human CDNF

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:500

Comment:

Negative Control: (ABIN7235051)

Blocking Peptide: (ABIN7235051)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CDNF

Target: CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))

Alternative Name: CDNF (CDNF Products)

Background: Cerebral dopamine neurotrophic factor, Arginine-rich mutated in early stage tumors-like 1, ARMET-like protein 1, ARMETL1,Neurotropic factors (NTFs) comprise an important group of secreted proteins regulating the life cycle of neurons during development. NTFs prevent apoptosis and control the number of neurons innervating a target tissue. NTFs also regulate migration, differentiation and maturation of postmitotic neuronal precursors and promote the regeneration of neurons after injury.Cerebral dopamine neurotrophic factor (CDNF) is a member of a sub-family of NTF proteins. The premature form of the protein consists of 187 amino acids while the mature form contains 161 residues. CDNF has a saposin-like N-terminal domain and an unstructured carboxy C-terminal domain with an intradomain cysteine bridge in a CXXC motif. Notably, saposin-like proteins interact with lipids, suggesting that CDNF's N-terminal may also interact with lipids. The saposin-like domain of CDNF crystalizes as a dimer at pH 4.6. It is presumed that CDNF functions upon synthesis and secretion since it does not contain prosequences for enzymatic activation.When CDNF is injected into the striatum of rats after the injection of 6-OHDA (6-OHDA leads to protracted retrograde degeneration of the nigrostriatal pathway) it significantly reduces amphetamine-induced rotational behavior and protects tyrosine hydroxylase (TH) positive dopaminergic cell bodies in the SN and TH-positive fibers in the striatum. In addition, CDNF had showed neurorestorative effects in the same animal model.Currently it is still unknown if CDNF also affects other types of neurons in the CNS or whether it has any effect on the PNS. It is also not clear to which target molecule CDNF binds and what signaling pathways it activates. CDNF might serve as a therapeutic agent for neurodegenerative diseases1.

Alternative names: CDNF, Cerebral dopamine neurotrophic factor, Arginine-rich mutated in early stage tumors-like 1, ARMET-like protein 1, ARMETL1

Gene ID: 441549

NCBI Accession: NM_001029954

UniProt: Q49AH0