CCR9 antibody (Extracellular)

Catalog No. ABIN7043036

Product Details anti-CCR9 Antibody

Target: CCR9 (Chemokine (C-C Motif) Receptor 9 (CCR9))

Binding Specificity: AA 205-218, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR9 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to CCR9 Chemokine Receptor

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 10,14 amino acid residues identical

Characteristics: Anti-CCR9 (extracellular) Antibody (ABIN7043036, ABIN7044177 and ABIN7044178)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CCR9 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DESTKLKSAVLTLK, corresponding to amino acid residues 205-218 of human CCR9

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CR029

Blocking Peptide: BLP-CR029

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CCR9

Target: CCR9 (Chemokine (C-C Motif) Receptor 9 (CCR9))

Alternative Name: CCR9 (CCR9 Products)

Background: C-C chemokine receptor type 9, CC-CKR-9, G-protein coupled receptor 28, GPR28, GPR-9-6, CDw199,CCR9 (C-C chemokine receptor type 9) belongs to the β-chemokine receptor family of the G-protein coupled receptor superfamily. Chemokine receptors play a crucial role in cell migration and are considered to be important therapeutic targets for inflammatory diseases and in tumor chemoresistance and metastasis. In addition, high expression of CCR9 has been reported in a wide range of cancers including melanoma, ovarian cancer, lung cancer, breast cancer and colon cancer. Thus, CCR9 is considered to be a potential tumor biomarker in diagnosis and therapy of cancer1,2. Under physiological conditions, CCR9 is mainly expressed in immature T lymphocytes and on the surface of intestinal cells2.CCR9 receptor interacts with its specific ligand- chemokine ligand 25 (CCL25, thymus-expressed chemokine) in a two-step binding mechanism in which the C-terminal region of CCL25 first binds the N-terminus region and extracellular loops of the receptor followed by the binding of the N-terminus of the chemokine. The two-step biding mechanism stabilizes the receptor in an active conformation that facilitates intracellular signaling1.

Alternative names: CCR9, C-C chemokine receptor type 9, CC-CKR-9, G-protein coupled receptor 28, GPR28, GPR-9-6, CDw199

Gene ID: 10803

NCBI Accession: NM_001256369

UniProt: P51686