CXCR5 antibody (Extracellular)

Catalog No. ABIN7043042

Product Details anti-CXCR5 Antibody

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Binding Specificity: AA 23-38, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to CXCR5 Chemokine Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity (Details): Will not recognize rat and mouse CXCR5.

Predicted Reactivity: Human only

Characteristics: Anti-Human CXCR5 (extracellular) Antibody is directed against an epitope of the human CXCR5. Anti-Human CXCR5 (extracellular) Antibody (ABIN7043042, ABIN7044168 and ABIN7044169)) can be used in western blot, immunocytochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize CXCR5 from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: RLDNYNDTSLVENHLC, corresponding to amino acid residues 23-38 of human CXCR5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-CR015

Blocking Peptide: BLP-CR015

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Alternative Name: CXCR5 (CXCR5 Products)

Background: C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, BLR1, Monocyte-derived receptor 15, MDR15, CD185,Chemokine receptors are a family of receptors that signal upon binding of one or more members of the chemokine superfamily of chemotactic cytokines. Chemokines coordinate development, differentiation and trafficking of various immune system cells making them key players of the innate and adaptive immune response.CXCR5 (also known as Burkitt lymphoma receptor 1) belongs to the CXC subfamily of chemokine receptors and is a G-coupled protein receptor with 7 transmembrane segments. There is considerable homology between CXCR5 and other members of the chemokine receptor family such as IL-9 receptor, CCR-1 and BLR2. CXCR5 expression is detected in peripheral blood CD4 and CD8 leukocytes as well as in secondary lymphatic organs and in granule and Purkinje layers in the brain. It is also expressed in Burkitt's lymphoma cells1.CXCR5 is activated by B-cell attracting chemokine 1 (BCA-1) that is chemotactic for human B lymphocytes. BCA-1 does not affect IL-2 stimulated T-cells, monocytes or neutrophils2. CXCR5 mediates correct T cell localization within granulomas, efficient macrophage activation and lymphoid follicle formation3.CXCR5 knockout mice have a severe defect in normal B cell migration and localization. Knockout mice lack inguinal lymph nodes, have a reduced amount of Peyer's patches and have abnormal primary lymphoid follicles and no functional germinal centers in the spleen4. Absence of CXCR5 has been implicated in increased susceptibility to tuberculosis to due defective T cell localization within the lung3.

Alternative names: CXCR5, C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, BLR1, Monocyte-derived receptor 15, MDR15, CD185

Gene ID: 643

NCBI Accession: NM_001716

UniProt: P32302