CX3CR1 antibody (Extracellular)

Catalog No. ABIN7043047

Product Details anti-CX3CR1 Antibody

Target: CX3CR1 (Chemokine (C-X3-C Motif) Receptor 1 (CX3CR1))

Binding Specificity: AA 7-20, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CX3CR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to CX3CR1 Receptor

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: Rat - identical

Characteristics: A Rabbit Polyclonal Antibody to CX3CR1 Receptor

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)ELDLENFEYDDSAE, corresponding to amino acid residues 7- 20 of mouse CX3CR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CR058

Blocking Peptide: BLP-CR058

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CX3CR1

Target: CX3CR1 (Chemokine (C-X3-C Motif) Receptor 1 (CX3CR1))

Alternative Name: CX3CR1 (CX3CR1 Products)

Background: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1,CX3C-chemokine receptor 1 (CX3CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors1.CX3CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX3C chemokine ligand 1, (CX3CL1). The major role of CX3CR1 in immune cells is to recognize and enter inflamed tissue according to CX3CL1 gradient and initiate the innate immune system2. The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)3. Expression and interaction of CX3CR1 with CX3CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).Both CX3CL1 and CX3CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX3CL1/CX3CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX3CL1/CX3CR1 signaling is associated with Alzheimer's Disease5. In the liver, CX3CR1 is expressed in monocytes, CD8+ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites4.

Alternative names: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1

Gene ID: 13051

NCBI Accession: NM_001337

UniProt: Q9Z0D9

Pathways: Cellular Response to Molecule of Bacterial Origin