CMKLR1 antibody (2nd Extracellular Loop)

Catalog No. ABIN7886251

The Rabbit Polyclonal anti-CMKLR1 antibody (ABIN7886251) specifically detects CMKLR1 in WB and IHC. The antibody is reactive with Rat samples.

Quick Overview for CMKLR1 antibody (2nd Extracellular Loop) (ABIN7886251)

Target: CMKLR1 (Chemokine-Like Receptor 1 (CMKLR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CMKLR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CMKLR1 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 201-215

Purpose: A Rabbit Polyclonal Antibody to Chemerin Receptor 1

Cross-Reactivity (Details): Will unlikely recognize CMKLR1 from human and mouse samples.

Predicted Reactivity: Rat only

Characteristics: Anti-Rat CMKLR1 (extracellular) Antibody (ABIN7043049, ABIN7044179 and ABIN7044180) is a highly specific antibody directed against an epitope of rat Chemerin receptor 1. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize the protein from rat samples only. The antibody is unlikely to recognize the receptor from human and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HSTHPRTDPVGYSRH, corresponding to amino acid residues 201-215 of rat Chemerin receptor 1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CMKLR1

Target: CMKLR1 (Chemokine-Like Receptor 1 (CMKLR1))

Alternative Name: CMKLR1

Background:

Synonyms: Chemokine-like receptor 1, Cmklr1, Chemerin1, Gpcr27, G-protein coupled receptor ChemR23, CHEMERINR, G-protein coupled chemoattractant-like receptor, G-protein coupled receptor DEZ

Description: Chemerin Receptor 1 belongs to class A G-protein coupled receptors. The receptor is coupled to Gi/o, leading to an increase in intracellular cAMP and Ca2+ following activation by the binding of Chemerin, its endogenous ligand. Like all GPCRs, it has seven transmembrane domains, an extracellular N-terminus and intracellular C-terminal tail1.Chemerin Receptor 1 has numerous names depending on how the gene encoding the receptor was cloned: Chemokine-like receptor 1, CMKLR1, ChemR23 and others all correspond to the same gene/protein1.Expression of Chemerin receptor 1 at the mRNA level is largely detected in adipose tissue, and skin dermal layer. At the protein level, CMKLR1 is expressed at the cell surface of dendritic cells, monocytes and macrophages, smooth muscle cells, adipose tissue, lung, lymph nodes and testis1.A role for the receptor in the inflammatory response has been attributed, however data indicate contradictory roles, proinflammatory and anti-inflammatory functions of the receptor. Expression of Chemerin receptor 1 in adipose tissue clearly establishes its involvement in energy metabolism as an important regulator of adipogenesis1.

Gene ID: 60669

NCBI Accession: NM_001142343

UniProt: O35786