CLIC4 antibody (Intracellular, N-Term)

Catalog No. ABIN7043057

The Rabbit Polyclonal anti-CLIC4 antibody is suitable to detect CLIC4 in samples from Human. It has been validated for WB, IF and IC.

Quick Overview for CLIC4 antibody (Intracellular, N-Term) (ABIN7043057)

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLIC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-CLIC4 Antibody

Binding Specificity: AA 8-20, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to CLIC4 Channel

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-CLIC4 Antibody (ABIN7043057 and ABIN7044140) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize CLIC4 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)NGLKEEDKEPLIE, corresponding to amino acid residues 8-20 of human CLIC4

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:200

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235106)

Blocking Peptide: (ABIN7235106)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CLIC4

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Alternative Name: CLIC4

Background:

Synonyms: Cl- intracellular channel 4, Intracellular chloride ion channel p64H1

Description: Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3.The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associated with the plasma membrane) via their single transmembrane domain1.When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend on whether CLICs are membrane bound or cytosolic1. For instance, CLIC4 is involved in keratinocyte differentiation in a TGFb-dependent manner4,5, in such a way that TGFb stimulation induces the expression and nuclear localization of CLIC4 and its subsequent interaction with transcription factors4. CLIC4 is also required for growth arrest and apoptosis under stress6.CLIC4 distribution is rather ubiquitous but shows higher expression level in the skin, kidney, brain and testes7.

Gene ID: 25932

NCBI Accession: NM_013943

UniProt: Q9Y696