CHRM4 antibody (AA 330-340)

Catalog No. ABIN7043064

This anti-CHRM4 antibody is a Rabbit Polyclonal antibody detecting CHRM4 in WB, IHC, IF and IC. Suitable for Rat.

Quick Overview for CHRM4 antibody (AA 330-340) (ABIN7043064)

Target: CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-CHRM4 Antibody

Binding Specificity: AA 330-340

Purpose: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M4

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 9,12 amino acid residues identical

Characteristics: Anti-CHRM4 Antibody (ABIN7043064, ABIN7044584 and ABIN7044585) is a highly specific antibody directed against an epitope of the rat M4 muscarinic receptor. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize M4 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C) RTLNPASKWSK, corresponding to amino acid residues 330-340 of rat M4 muscarinic receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235125)

Blocking Peptide: (ABIN7235125)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CHRM4

Target: CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))

Alternative Name: CHRM4

Background: Muscarinic acetylcholine receptor M4, Cholinergic receptor muscarinic 4, mAChR M4, HM4,Acetylcholine is a neurotransmitter which activates two different groups of receptors: nicotinic acetylcholine receptors (nAChR) which belong to the superfamily of ligand-gated ion channels, and muscarinic acetylcholine receptors (mAChR) which belong to the G-protein coupled receptor (GPCR) superfamily. There are five separate gene products (M1-M5) that, assembled, make up this GPCR subfamily.Generally, M1, M3 and M5 muscarinic receptors are known to activate phospholipase C (PLC) via Gq coupling, while M2 and M4 muscarinic receptors couple to Gi/o, and therefore inhibit adenylate cyclase1. This classification is however not clear cut. mAChR can activate adenylate cyclase by coupling to Gs2. In addition, M2 and M4 muscarinic receptors, when overexpressed, can also activate adenylate cyclase in some systems3-5.Evidence suggests that muscarinic receptors can form homo- or heterodimers and that the dimer formed can subsequently affect the downstream signaling pathways1.Muscarinic receptors have been shown to regulate voltage-gated Ca2+ channels, namely CaV2.16, as well acid sensing ion channels (ASIC)7. Generally speaking, the actions of muscarinic receptors on ion channels can be either via 2nd messengers or through their direct action on the channels once activated8.Furthermore, muscarinic receptors can also promote endocytosis of ion channels (KV1.2 for example) by recruiting tyrosine kinases that phosphorylate the channel in order to terminate its activity9. Other diverse and important functions of muscarinic receptors include cell growth, survival and physiology1.Expression of muscarinic receptors is found in neurons in the central nervous system as well as in the peripheral nervous system. These receptors are also expressed in cardiac and smooth muscle, lung, intestine, ovary and urothelium1,10. M4 muscarinic receptor is mostly expressed in the striatum where it colocalizes with D1 dopamine receptors11.Malfunction of M4 muscarinic receptor is associated with a number of psychological and neurodegenerative diseases like Schizophrenia, Alzheimer's and Parkinson's diseases12.

Alternative names: M4 Muscarinic Receptor, CHRM4, Muscarinic acetylcholine receptor M4, Cholinergic receptor muscarinic 4, mAChR M4, HM4

Gene ID: 25111

NCBI Accession: NM_000741

UniProt: P08485

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Synaptic Membrane