C5AR1 antibody (Extracellular, N-Term)

Catalog No. ABIN7043082

The Rabbit Polyclonal anti-C5AR1 antibody (ABIN7043082) specifically detects C5AR1 in WB, FACS and LCI. The antibody is reactive with Human samples.

Quick Overview for C5AR1 antibody (Extracellular, N-Term) (ABIN7043082)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C5AR1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-C5AR1 Antibody

Binding Specificity: AA 16-28, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor

Predicted Reactivity: Human only Not recommended for use with rat,mouse samples

Characteristics: Anti-Human C5aR1 (extracellular) Antibody (ABIN7043082 and ABIN7043927) is a highly specific antibody directed against an epitope of human C5a anaphylatoxin receptor. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize C5aR from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DKDTLDLNTPVDK, corresponding to amino acid residues 16-28 of human C5a anaphylatoxin receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: C5AR1

Background:

Synonyms: C5a1 receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5R1, Complement component 5a receptor 1, CD88

Description: The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.C5a is one of the most potent inflammatory peptides generated by complement activation. This peptide has many functions such as acting as a chemoattractant for neutrophils2,3. C5a acts as a vasodilator3,4 and activates the coagulation pathway3,5 just to name a few.C5a exerts its numerous actions by binding to C5aR and C5L2. C5aR belongs to the superfamily of G-protein coupled receptors (GPCRs). Like all members, C5aR has seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. C5aR can form homodimers6-8 or heteromer with CCR5, chemokine receptor, for example8,9. Activation of C5aR by C5a binding causes Ca2+ mobilization10.C5aR expression is detected in neutrophils, eisinophils, basophils and monocytes3,11,12. The receptor is also expressed in lung and liver13,14.Blocking C5aR by peptides or organic small molecules is in the pipeline for treating chronic inflammatory disorders/diseases like arthritis, inflammatory bowel disease and lung/liver injuries8.

Gene ID: 728

NCBI Accession: NM_001736

UniProt: P21730

Pathways: Complement System