CNTNAP2 antibody (Intracellular)

Catalog No. ABIN7043084

Product Details anti-CNTNAP2 Antibody

Target: CNTNAP2 (Contactin Associated Protein-Like 2 (CNTNAP2))

Binding Specificity: AA 1315-1331, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNTNAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Caspr2

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: Anti-Caspr2 Antibody is directed against an epitope of human Caspr2. Anti-Caspr2 Antibody (ABIN7043084, ABIN7045145 and ABIN7045146)) can be used in western blot and immunohistochemistry analysis. It has been designed to recognize Caspr2 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DPNFTETIDESKKEWLI, corresponding to amino acid residues 1315-1331 of human Caspr2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PZ005

Blocking Peptide: BLP-PZ005

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CNTNAP2

Target: CNTNAP2 (Contactin Associated Protein-Like 2 (CNTNAP2))

Alternative Name: CNTNAP2 (CNTNAP2 Products)

Background: Contactin-associated protein-like 2, CNTNAP2,Contactin-associated protein 2 (Caspr2) is a member of the neurexin superfamily, a group of transmembrane proteins that mediate cell-to-cell interactions in the nervous system1,2.Neurexins are adhesion molecules expressed mainly at presynaptic locations that form trans-synaptic cell-to-cell adhesion complexes via binding to their postsynaptic partners, the neuroligins2.Caspr2, like other neurexin proteins, is a type I membrane protein that contains epidermal growth factor repeats, laminin G domains, an F5/8 type C domain, and fibrinogen-like domains in its extracellular domain1,2.Caspr2 is localized at the juxtaparanodes of myelinated axons, a specialized region that mediates interactions between neurons and glia during nervous system development1.The juxtaparanodal region is highly enriched with heteromultimers of the K+ channels KV1.1, KV1.2, and their cytoplasmic KVβ2 subunit, which may help in axon conduction stabilization and the maintenance of the internodal resting potential1. Caspr2 is essential for the targeted localization of these channels in the juxtaparanodal regions. Indeed, targeted disruption of Caspr2 resulted in a marked reduction in the accumulation of K+ channels at the juxtaparanodes in both peripheral and central nervous system axons3.Moreover, mutations in the Caspr2 gene have been implicated in multiple neurodevelopmental disorders, including Tourette syndrome, schizophrenia, epilepsy, autism, attention-deficit hyperactivity disorder and mental retardation4-6.

Alternative names: Caspr2, Contactin-associated protein-like 2, CNTNAP2

Gene ID: 26047

NCBI Accession: NM_014141

UniProt: Q9UHC6