CNTNAP2 antibody (Extracellular Domain)

Catalog No. ABIN6656679

Product Details anti-CNTNAP2 Antibody

Target: CNTNAP2 (Contactin Associated Protein-Like 2 (CNTNAP2))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNTNAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-CASPR2 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with CASPR2 from Mouse, Human, and Rat based on 100% homology with the immunizing sequence. No cross reactivity to CASPR/Paranodin. Cross-reactivity with CASPR2 from other sources has not been determined. Scaffolds research.

Immunogen:

Immunogen: CASPR2 Antibody was produced in mice by repeated immunizations with a fusion protein (extracellular domain) of human CASPR2.

Immunogen Type: Recombinant Protein

Clone: S67-25

Isotype: IgG2a

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-CASPR2 Antibody is suitable for use in WB, IP, and IHC. Expect a band approximately ~180 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 1X PBS, pH 7.4

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for CNTNAP2

Target: CNTNAP2 (Contactin Associated Protein-Like 2 (CNTNAP2))

Alternative Name: CASPR2 (CNTNAP2 Products)

Synonyms: AUTS15 antibody, CASPR2 antibody, CDFE antibody, NRXN4 antibody, PTHSL1 antibody, 5430425M22Rik antibody, Caspr2 antibody, mKIAA0868 antibody, CASPR antibody, MGC145769 antibody, MGC147944 antibody, MGC147981 antibody, contactin associated protein like 2 antibody, contactin associated protein-like 2 antibody, contactin associated protein like 2b antibody, CNTNAP2 antibody, Cntnap2 antibody, cntnap2b antibody, cntnap2 antibody

Background:

Synonyms: Cntnap2, CNTP2, Contactin associated protein like 2, Cell recognition molecule Caspr2, CASPR2, KIAA0868

Background: Caspr (contactin-associated protein) is a part of the neurexin family. It lies in the paranodal section of the myelin sheath. It's role is for myelin sheath attachment along with contactin in a cis-complex (3). Caspr and Caspr2 regulate the formation of distinct axonal domains around the nodes of Ranvier. Caspr is required for the generation of a membrane barrier at the paranodal junction, whereas Caspr serves as a membrane scaffold that clusters Kv1 channels at the juxtaparanodal region. Both interact with protein 4.1B.

Gene Name: CNTNAP2

Gene ID: 26047

NCBI Accession: NP_054860

UniProt: Q9UHC6