CNGA3 antibody (Intracellular)

Catalog No. ABIN7043091

Product Details anti-CNGA3 Antibody

Target: CNGA3 (Cyclic Nucleotide Gated Channel alpha 3 (CNGA3))

Binding Specificity: AA 594-611, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNGA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to CNGA3 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Recognizes the two published isoforms of CNGA3.

Predicted Reactivity: Mouse - 15,18 amino acid residues identical

Characteristics: Anti-CNGA3 Antibody (ABIN7043091 and ABIN7044975)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CNGA3 from rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GHGFSPDRENSEDASKAD, corresponding to amino acid residues 594-611 of rat CNGA3 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: ICC

Negative Control: BLP-PC060

Blocking Peptide: BLP-PC060

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CNGA3

Target: CNGA3 (Cyclic Nucleotide Gated Channel alpha 3 (CNGA3))

Alternative Name: CNGA3 (CNGA3 Products)

Background: Cyclic nucleotide-gated cation channel alpha 3, CNG channel alpha 3, CNG3, CNCG3, CNGα2, Cone photoreceptor cGMP-gated channel subunit alpha, CCNC1, ACHM2,Cyclic nucleotides are important second messengers in many cellular functions such as visual transduction, and relaxation of smooth muscle cells. Cyclic nucleotides exert their cellular functions through three major classes of cellular receptors, one of them is the cyclic nucleotide-gated (CNG) channels.1 The CNG channels are non-selective cation channels facilitating the influx of Na+ and Ca2+ ions, following activation by intracellular cyclic nucleotides.2 In vertebrates, six members of the CNG channel family were identified and grouped according to sequence homology into two subtypes, CNGA and CNGB. The native CNG channels are composed of a and b subunits in a tetrameric configuration. To date, four types of the a subunits (CNGA1-4) and two b subunits (CNGB1, CNGB3) have been characterized.3-4 Each subunit contains 6 TM domains and intracellular cAMP or cGMP binding domains but, are also modulated by other factors including phosphorylation and calmodulin.5 In a heterologous expression system, only the a subunits are capable of forming functional homomeric channels. CNG ion channels are essential in visual and olfactory signal transduction. The CNGA3 channel is primarily expressed in cone photoreceptors where it participates in light transduction (daylight and color vision). CNG channels expression was also found in the hippocampus, cortex, Purkinje cells and other neural derived tissues. CNGA3 was also detected in kidney, heart, brain and sperm, where it was thought to have an important role in the control of sperm motility and fertility. However, CNGA3-deficient mice were shown to be as fertile and produce at normal rate as wild types.6

Alternative names: CNGA3, Cyclic nucleotide-gated cation channel alpha 3, CNG channel alpha 3, CNG3, CNCG3, CNGalpha2, Cone photoreceptor cGMP-gated channel subunit alpha, CCNC1, ACHM2

Gene ID: 85257

NCBI Accession: NM_001298

UniProt: Q9QWN7

Pathways: Phototransduction