CNGA4 antibody (Intracellular)

Catalog No. ABIN7043092

Product Details anti-CNGA4 Antibody

Target: CNGA4 (Cyclic Nucleotide Gated Channel alpha 4 (CNGA4))

Binding Specificity: AA 2-19, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNGA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to CNGA4 Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 15,18 amino acid residues identical

Characteristics: Anti-CNGA4 Antibody (ABIN7043092 and ABIN7044985)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CNGA4 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C) SQDGKVKTTESTPPAPTK, corresponding to amino acid residues 2-19 of rat CNGA4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PC074

Blocking Peptide: BLP-PC074

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CNGA4

Target: CNGA4 (Cyclic Nucleotide Gated Channel alpha 4 (CNGA4))

Alternative Name: CNGA4 (CNGA4 Products)

Background: Cyclic nucleotide-gated cation channel alpha-4, CNG4, Cyclic nucleotide-gated olfactory channel subunit OCNC2, OCNCb,Cyclic Nucleotide-Gated (CNG) channels belong to the superfamily of voltage-gated ion channels. Although permeable to various ions such as the monovalent Na+ and K+ ions, and the divalent Ca2+ ion, they are gated by the intracellular binding of the cyclic nucleotides cAMP and cGMP and not by voltage per se (CNGs bind preferably to cGMP)1.Six channels form this subfamily: The A subunit (CNGA1-4) and the B subunit (CNGB1 and CNGB3). Functional entities are formed by the assembly of four subunits. Each subunit consists of six membrane spanning domains, where the fourth transmembrane domain is highly positively charged, a pore domain between transmembrane domains five and six, and intracellular N- and C-termini. The cyclic nucleotide binding domain is located in the C-terminal region and is responsible for the channel gating upon cyclic nucleotide binding1.These channels are highly expressed in retinal photoreceptors and olfactory neurons where their role has been extensively studied2. CNG channels have also been detected in brain, testis and kidney although their role in these tissues has yet to be unraveled2. When heterologously expressed all subunits with exception of CNGA4, CNGB1 and CNGB3 can form functional homomeric channels. In olfactory sensory neurons, CNG channels which are formed by two CNGA2, one CNGA4 subunits and an isoform of CNGB1, are activated by the binding of cAMP, whose levels increase in response to the binding of odorant molecules to their respective receptors1,3.CNGA4 knockout mice are fertile and morphologically similar to their wild type littermates4,5. In these mice, the olfactory bulb and epithelium are developed but these mice display defects in odor adaptation, and at the molecular level, they are less sensitive to cAMP activation6.

Alternative names: CNGA4, Cyclic nucleotide-gated cation channel alpha-4, CNG4, Cyclic nucleotide-gated olfactory channel subunit OCNC2, OCNCb

Gene ID: 85258

NCBI Accession: NM_001037329

UniProt: Q64359