CasLTR2 antibody (Extracellular)

Catalog No. ABIN7043095

Product Details anti-CasLTR2 Antibody

Target: CasLTR2 (CYSLTR2) (Cysteinyl Leukotriene Receptor 2 (CYSLTR2))

Binding Specificity: AA 270-284, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CasLTR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to Human CysLTR2

Specificity: 3rd extracellular loop

Cross-Reactivity (Details): Not recommended for use with rat and mouse samples.

Predicted Reactivity: Human only

Characteristics: Anti-Human CysLTR2 (extracellular) Antibody (ABIN7043095 and ABIN7044560)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CysLTR2 from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HLTTWKVGL(S)KDRLH, corresponding to amino acid residues 270-284 of human CysLTR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-LR004

Blocking Peptide: BLP-LR004

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CasLTR2

Target: CasLTR2 (CYSLTR2) (Cysteinyl Leukotriene Receptor 2 (CYSLTR2))

Alternative Name: CYSLTR2 (CYSLTR2 Products)

Background: CYSLT2, Cysteinyl leukotriene receptor 2, hGPCR21, G-protein coupled receptor HG57,LTC4, LTD4, and LTE4 are cysteinyl leukotrienes (cys-LTs) peptide conjugated lipids derived from arachidonic acid as a result of immune or inflammatory stimuli1-3.The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also named cysLT1 and cysLT2 respectively). There is evidence though that other receptors are yet to be identified4. These two receptors couple to both pertussis toxin sensitive (Gi/o) and insensitive (Gq, which initiates Ca2+ influx) G-proteins for their signaling. The G-protein coupled to is probably determined by which ligand binds the receptor as all three cys-LTs can activate the two receptors albeit with different affinities5. In mouse, CysLTR1 and CysLTR2 are subject to alternative splicing. CysLTR1 receptor expresses greater affinity for LTD4 and binds equally to LTC4 and LTE4whereas CysLTR2 has greater and equal affinity towards LTC4 and LTD4 over LTE45.Human CysLTR1 is highly expressed in spleen, peripheral blood, leukocytes. It is less strongly expressed in lung, small intestine, pancreas and hardly or not expressed in liver, colon, kidney, skeletal muscle, testis heart and brain4. On the other hand, CysLTR2 is highly expressed in spleen and leukocytes. Its expression in the heart, brain, spinal cord and adrenal gland differs it from that of CysLTR14.Cys-LTs and their receptors are involved in respiratory diseases like asthma and other conditions such as cancer cardiovascular, gastrointestinal, skin and immune disorders1,6.

Alternative names: CysLTR2, CYSLT2, Cysteinyl leukotriene receptor 2, hGPCR21, G-protein coupled receptor HG57

Gene ID: 57105

NCBI Accession: NM_001308465

UniProt: Q9NS75