DRD4 antibody (3rd Intracellular Loop)

Catalog No. ABIN7043110

This Rabbit Polyclonal antibody specifically detects DRD4 in WB. It exhibits reactivity toward Rat.

Quick Overview for DRD4 antibody (3rd Intracellular Loop) (ABIN7043110)

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DRD4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DRD4 Antibody

Binding Specificity: 3rd Intracellular Loop, AA 215-228

Purpose: A Rabbit Polyclonal Antibody to D4 Dopamine Receptor

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 9,14 amino acid residues identical

Characteristics: Anti-D4 Dopamine Receptor Antibody is directed against an epitope located at the 3rd intracellular loop of the rat D4 dopamine receptor. Anti-D4 Dopamine Receptor Antibody (ABIN7043110 and ABIN7044233) can be used in western blot analysis, and recognizes DRD4 from rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RRWEAARHTKLHSR, corresponding to amino acid residues 215-228 of rat DRD4 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235246)

Blocking Peptide: (ABIN7235246)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for DRD4

Target: DRD4 (Dopamine Receptor D4 (DRD4))

Alternative Name: DRD4

Background:

Synonyms: DRD4, D4DR, D(2C) dopamine receptor

Description: The dopamine neurotransmitter belongs to the catecholamines and can therefore be further converted into adrenaline and noreadrenaline. Dopamine has various physiological roles, including learning and memory, motor output and endocrine regulation. It does so by binding and activating dopamine receptors which belong to the G-protein coupled receptor superfamily (GPCR)1.The D4 dopamine receptor (DRD4) belongs to the D2-like family as do D2 and D3 dopamine receptors. Like all GPCRs, it has seven transmembrane spanning membrane regions. Structure-wise, members of the family share high homology in the transmembrane domains and lower homology in the extracellular N-terminal and the intracellular C-terminal domains. Notably, the coding region of the 3rd intracellular loop of DRD4 is known to undergo extensive polymorphism2.Like many GPCRs, each dopamine receptor subtype can react with more than one G-protein giving rise to different signaling possibilities3. Whereas D2-like dopamine receptors are generally considered to couple to Gi, and therefore inhibit adenylyl cyclase, the signaling through DRD4 is complicated due to the polymorphisms in the 3rd intracellular loop. It seems that this region is important to G-coupling as different polyphormisms in the region influence the ability of DRD4 to couple to adenylyl cyclase and G-proteins4,5. D4 dopamine receptors also influence Ca2+ levels3,6. They could also interact with G-protein couple inwardly rectifying K+ channel to ultimately cause a decrease in the firing rate of neurons7.The distribution of DRD4 mostly includes the brain and is mainly found post-synaptically in dendritic shafts and spines of mammalian striatum8.

Gene ID: 25432

NCBI Accession: NM_000797

UniProt: P30729

Pathways: cAMP Metabolic Process, Synaptic Membrane, Proton Transport, Photoperiodism, Negative Regulation of Transporter Activity