EFCAB4B antibody (Intracellular)

Catalog No. ABIN7043114

The Rabbit Polyclonal anti-EFCAB4B antibody has been validated for WB. It is suitable to detect EFCAB4B in samples from Human.

Quick Overview for EFCAB4B antibody (Intracellular) (ABIN7043114)

Target: EFCAB4B (EF-Hand Calcium Binding Domain 4B (EFCAB4B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EFCAB4B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EFCAB4B Antibody

Binding Specificity: AA 245-258, Intracellular

Purpose: A Rabbit Polyclonal Antibody to CRAC Channel Regulator 2A

Specificity: Intracellular

Cross-Reactivity: Human, Rat

Predicted Reactivity: Rat - 13,14 amino acid residues identical

Characteristics: Anti-CRACR2A (EFCAB4B) Antibody is directed against an epitope of the human CRAC channel regulator 2A. Anti-CRACR2A (EFCAB4B) Antibody (ABIN7043114, ABIN7044115 and ABIN7044116) can be used in western blot analysis. It has been designed to recognize CRAC channel regulator 2A from rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KSEKEQFLLKDTER, corresponding to amino acid residues 245-258 of human CRACR2A

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235259)

Blocking Peptide: (ABIN7235259)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for EFCAB4B

Target: EFCAB4B (EF-Hand Calcium Binding Domain 4B (EFCAB4B))

Alternative Name: CRACR2A

Background: CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B,Calcium is a vital ion for the functioning of many intracellular processes. When Ca2+ stored in the endoplasmic reticulum is depleted, there is an activation of store-operated channels that results in the influx of Ca2+ from outside the cell. In non-excitable cells and especially in immune cells, most of this influx is carried out by calcium release activated calcium channels also known as "CRACs"1.Mediation of signals from the ER to CRACs and activation of the CRAC channel is conducted by two modulators that physically cluster with the CRAC channel known as Orai1 and STIM1. STIM1 is a single transmembrane segment protein that has a long C-terminal cytoplasmic region and an N-terminus which detects ER Ca2+ depletion. Orai1 is a macromolecular complex with linkers of varying lengths between the ER and plasma membranes.Novel findings suggest the existence of a complex comprised of Orai1, STIM1 and additional proteins. One of these proteins is an EF-hand protein named CRACR2A which regulates Orai-STIM interaction. An EF-hand is a structural domain found in Ca2+ binding proteins consisting of two alpha helices linked by a short loop region.CRACR2A interacts with the cytoplasmic regions of both Orai1 and STIM1 thus creating a tertiary complex. Under high concentrations of Ca2+, CRACR2A caused a dissociation of Orai1 and STIM1 and a mutated CRACR2A was found, in one study, to cause elevated Ca2+ levels that caused cell death in T cells2.Further research is required to fully determine the activation and regulation mechanisms of CRACs and this can be performed by better understanding CRACR2A and its binding proteins.

Alternative names: CRACR2A (EFCAB4B), CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B

Gene ID: 84766

NCBI Accession: NM_032680

UniProt: Q9BSW2