F4/80 antibody (Extracellular)

Catalog No. ABIN7043117

The Guinea Pig Polyclonal anti-F4/80 antibody is suitable to detect F4/80 in samples from Human. It has been validated for WB.

Quick Overview for F4/80 antibody (Extracellular) (ABIN7043117)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivity: Human

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This F4/80 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-F4/80 Antibody

Binding Specificity: AA 58-72, Extracellular

Purpose: A Guinea Pig Polyclonal Antibody to EMR1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,Rat - 11,14 amino acid residues identical

Characteristics: Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody raised in guinea pig, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize ADGRE1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-EMR1 (ADGRE1) (extracellular) Antibody (ABIN7043115, ABIN7044269 and ABIN7044270) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235262)

Blocking Peptide: (ABIN7235262)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: ADGRE1

Background: MR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480,Human epidermal growth factor (EGF)-like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2.EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1.Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.

Alternative names: MR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480

Gene ID: 2015

NCBI Accession: NM_001256252

UniProt: Q14246