Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Ephrin A2 antibody (Extracellular)

EFNA2 Reactivity: Mouse WB, IF, FACS, IHC, LCI Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043129
  • Target See all Ephrin A2 (EFNA2) Antibodies
    Ephrin A2 (EFNA2)
    Binding Specificity
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 71-84, Extracellular
    Reactivity
    • 27
    • 19
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 21
    • 6
    • 1
    Rabbit
    Clonality
    • 22
    • 6
    Polyclonal
    Conjugate
    • 21
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Ephrin A2 antibody is un-conjugated
    Application
    • 19
    • 9
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)
    Purpose
    A Rabbit Polyclonal Antibody to Ephrin-A2
    Specificity
    Extracellular
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Rat - identical, human - 13,14 amino acid residues identical
    Characteristics
    Anti-Ephrin-A2 (extracellular) Antibody (ABIN7043129, ABIN7044257 and ABIN7044258) is a highly specific antibody directed against an epitope of mouse EFNA2. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize EFNA2 from rat, mouse, and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)HYGAPLPPAERMER, corresponding to amino acid residues 71-84 of mouse Ephrin-A2

    Isotype
    IgG
    Top Product
    Discover our top product EFNA2 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

    Application Dilutions Western blot wb: 1:400

    Comment

    Negative Control: (ABIN7235305)

    Blocking Peptide: (ABIN7235305)

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    Ephrin A2 (EFNA2)
    Alternative Name
    EFNA2 (EFNA2 Products)
    Background
    EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2,Eph receptor tyrosine kinases family and their ligands, ephrins, are membrane proteins that primarily regulate cell to cell repulsion and cell adhesion and movement by modulating the organization of the actin cytoskeleton mainly through Rho family GTPases. Eph receptors and ephrin ligand signaling regulaes many aspects of cell interaction in the developing neocortex, including migration and axonal target recognition. Ephrin signaling plays an important role in guiding neurons and their projections during embryonic development1,2.In mammals, the Eph receptor tyrosine kinases family has 14 members that are divided into 2 subclasses, the EphA (A1-A8 and A10) and EphB (B1-B4 and B6). This division is based on the sequence similarity of their extracellular domains. EphA receptors bind to glycosylphosphatidyl inositol-anchored ephrin-A ligands, and EphB receptors bind to transmembrane ephrin-B proteins. The ephrin-A proteins include five members ephrin-A1 to ephrin-A5 whereas ephrin-B proteins include three ephrin-B1 to ephrin-B32,3.Ephrin-A2 protein is abundantly expressed in retinal axons. Mutations in Ephrin-A2 disrupts topographic ordering of projections in the developing visual system. A knockout of ephrin-A2 in adult mice shows focal patches of disorganized neocortical laminar architecture, ranging in severity from reduced neuronal density to a complete lack of neurons1,2.

    Alternative names: Ephrin-A2, EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2
    Gene ID
    13637
    NCBI Accession
    NM_001405
    UniProt
    P52801
You are here:
Support