Ephrin A2 antibody (Extracellular)

Catalog No. ABIN7043129

Product Details anti-Ephrin A2 Antibody

Target: Ephrin A2 (EFNA2)

Binding Specificity: AA 71-84, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Ephrin-A2

Specificity: Extracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-Ephrin-A2 (extracellular) Antibody (ABIN7043129, ABIN7044257 and ABIN7044258) is a highly specific antibody directed against an epitope of mouse EFNA2. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize EFNA2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HYGAPLPPAERMER, corresponding to amino acid residues 71-84 of mouse Ephrin-A2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235305)

Blocking Peptide: (ABIN7235305)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Ephrin A2

Target: Ephrin A2 (EFNA2)

Alternative Name: EFNA2 (EFNA2 Products)

Background: EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2,Eph receptor tyrosine kinases family and their ligands, ephrins, are membrane proteins that primarily regulate cell to cell repulsion and cell adhesion and movement by modulating the organization of the actin cytoskeleton mainly through Rho family GTPases. Eph receptors and ephrin ligand signaling regulaes many aspects of cell interaction in the developing neocortex, including migration and axonal target recognition. Ephrin signaling plays an important role in guiding neurons and their projections during embryonic development1,2.In mammals, the Eph receptor tyrosine kinases family has 14 members that are divided into 2 subclasses, the EphA (A1-A8 and A10) and EphB (B1-B4 and B6). This division is based on the sequence similarity of their extracellular domains. EphA receptors bind to glycosylphosphatidyl inositol-anchored ephrin-A ligands, and EphB receptors bind to transmembrane ephrin-B proteins. The ephrin-A proteins include five members ephrin-A1 to ephrin-A5 whereas ephrin-B proteins include three ephrin-B1 to ephrin-B32,3.Ephrin-A2 protein is abundantly expressed in retinal axons. Mutations in Ephrin-A2 disrupts topographic ordering of projections in the developing visual system. A knockout of ephrin-A2 in adult mice shows focal patches of disorganized neocortical laminar architecture, ranging in severity from reduced neuronal density to a complete lack of neurons1,2.

Alternative names: Ephrin-A2, EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2

Gene ID: 13637

NCBI Accession: NM_001405

UniProt: P52801