Ephrin B1 antibody (Extracellular)

Catalog No. ABIN7043131

This Rabbit Polyclonal antibody specifically detects Ephrin B1 in WB, FACS and LCI. It exhibits reactivity toward Human.

Quick Overview for Ephrin B1 antibody (Extracellular) (ABIN7043131)

Target: Ephrin B1 (EFNB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Ephrin B1 Antibody

Binding Specificity: AA 208-221, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Ephrin-B1

Specificity: Extracellular domain

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Mouse - identical, rat - 13,14 amino acid residues identical

Characteristics: Anti-Ephrin-B1 (extracellular) Antibody is directed against an epitope of human Ephrin-B1. Anti-Ephrin-B1 (extracellular) Antibody (ABIN7043131, ABIN7044261 and ABIN7044262) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the ligand in living cells. It has been designed to recognize Ephrin-B1 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KHETVNQEEKSGPG, corresponding to amino acid residues 208-221 of human Ephrin-B1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235313)

Blocking Peptide: (ABIN7235313)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Ephrin B1

Target: Ephrin B1 (EFNB1)

Alternative Name: EFNB1

Background: EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1,The erythropoietin-producing hepatocellular (EPH) receptors represent the largest known family of receptor tyrosine kinases and are activated by interactions with cell-surface ligands, termed EFNs. EPH receptors have been classified into two subfamilies, EPHA and EPHB, according to their preference for either glycosylphosphatidylinositol (GPI)-anchored EFN-A ligands or transmembrane EFN-B ligands. Thus far, 14 vertebrate receptors and eight ligands have been identified1. All the ligands share a conserved core sequence of approximately 125 amino acids, including 4 invariant cysteine residues, probably corresponding to a receptor binding domain. This is followed by a membrane anchorage domain, taking the form of a GPI anchor for five of the ligands (ephrin-A1 to -A5) or a transmembrane domain for three of them (ephrin-B1 to -B3) 2.Ephrin-B1 is signals forward or reverse signaling mechanisms. Ephrin-B1 PDZ-dependent reverse signaling controls axon guidance of the corpus callosum, whereas forward signaling is critical for normal craniofacial development2.Eph receptors and ephrins are highly expressed in the brain and in the developing nervous system, where they are involved in fundamental developmental processes of the nervous system, including axon guidance, axonfasciculation, neural crest cell migration, acquisition of brain subregional identity,and neuronal cell survival. Ephrins are dramatically expressed in a wide range of regions of the vertebrate embryo including the ectoderm, mesoderm, and endoderm3.Overexpression of B-type ephrin in cancer cells is reported to correlate with high invasion and high vascularity of tumors and elevated expression of ephrin-B1 is observed in poorly differentiated invasive tumor cells and other tumors with poor clinical prognosis4.

Alternative names: Ephrin-B1, EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1

Gene ID: 1947

NCBI Accession: NM_004429

UniProt: P98172

Pathways: RTK Signaling