PIEZO2 antibody (AA 1092-1104)

Catalog No. ABIN7043133

Product Details anti-PIEZO2 Antibody

Target: PIEZO2 (FAM38B) (Family with Sequence Similarity 38, Member B (FAM38B))

Binding Specificity: AA 1092-1104

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIEZO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Piezo2

Specificity: Located in an internal region of human Piezo2 (the exact topology of Piezo2 remains to be defined).

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 12,13 amino acid residues identical

Characteristics: Anti-Piezo2 Antibody (ABIN7043133, ABIN7044990 and ABIN7044991) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize Piezo2 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RTIFHDITRLHLD, corresponding to amino acid residues 1092 - 1104 of human Piezo2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC|Whole Tissue Staining

Negative Control: (ABIN7235322)

Blocking Peptide: (ABIN7235322)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PIEZO2

Target: PIEZO2 (FAM38B) (Family with Sequence Similarity 38, Member B (FAM38B))

Alternative Name: PIEZO2 (FAM38B Products)

Background: Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B,Piezo-type mechanosensitive ion channel component 2 (Piezo2) is a homotrimeric ion channel. Piezo2 depicts rapidly adapting mechanically activated (MA) currents in somatosensory neurons.Piezo1 and Piezo2 comprise the Piezo protein family. These are large transmembrane proteins are conserved among various species, and all have between 24 and 36 predicted transmembrane domains. Both channels open in response to mechanical stimuli, allowing positively charged ions, including calcium, to flow into the cell1.Piezo2 protein contains over 2,800 residues, it forms a three-bladed, propeller like trimer that comprises 114 transmembrane helices (38 per protomer). Transmembrane helices 1-36 (TM1-36) are folded into nine tandem units of four transmembrane helices each to form the unusual non-planar blades2.Piezo2 has an essential role in sensing gentle touch, tactile pain, airway stretch and lung inflation. It also serves as a baroreceptor for sensing blood pressure and regulating heart rate. Mutations in human Piezo2 are associated with distal arthrogryposis, a disorder characterized by joint deformities (contractures) that restrict movement in the hands and feet3,4.

Alternative names: Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B

Gene ID: 63895

NCBI Accession: NM_022068

UniProt: Q9H5I5