FPR1 antibody (Extracellular)

Catalog No. ABIN7043135

Product Details anti-FPR1 Antibody

Target: FPR1 (Formyl Peptide Receptor 1 (FPR1))

Binding Specificity: AA 179-192, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FPR1 antibody is un-conjugated

Application: Western Blotting (WB), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to Human FPR1

Specificity: 2nd extracellular loop

Predicted Reactivity: Human only Not recommended for use with rat,mouse samples

Characteristics: Anti-Human FPR1 (extracellular) Antibody (ABIN7043135, ABIN7044275 and ABIN7044276)) is a highly specific antibody directed against an epitope of the human N-formyl peptide receptor 1. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize FPR1 from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NFSPWTNDPKERIN, corresponding to amino acid residues 179-192 of human FPR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-FR001

Blocking Peptide: BLP-FR001

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for FPR1

Target: FPR1 (Formyl Peptide Receptor 1 (FPR1))

Alternative Name: FPR1 (FPR1 Products)

Background: N-formyl peptide receptor 1, fMLP receptor,Chemotactic factors from both Gram-positive and Gram-negative bacteria are short peptides with N-formyl methionine at the N-terminus (extensively reviewed in reference 1). These peptides are released from bacteria during infection and activate formyl peptide receptors (FPR), members of the G-protein coupled receptor (GPCR) superfamily. In humans, the FPR family consists mainly of three receptors, FPR1, FPR2/ALX (formerly FPRL1), and FPR3 (formerly FPRL2) which all couple to the Gi subtype of G-proteins and ultimately lead to the activation of phospholipase C and intracellular Ca2+ increase1,2.FPRL1, or FPR2/ALX as it is commonly called, is a seven transmembrane protein like all GPCRs. This receptor was originally cloned by screening a HL60 neutrophil cDNA library with a FPR1 cDNA probe3. FPR2/ALX shares 69 % identity with FPR1 and despite its high homology, it displays relatively low affinity for fmlf, the most potent N-formyl peptide released by bacteria3.FPR1 was originally found in neutrophils and later found to be distributed in myeloid and non-myeloid cells as is the case for FPR2/ALX and FPR3 (FPR3 though is not expressed in neutrophils). FPR1 is also expressed in multiple organs and tissues including epithelial cells in organs with secretory functions, endocrine cells, liver hepathocytes, smooth muscle cells and endothelial cells, brain spinal cord and both motor and sensory neurons4. FPR2/ALX has a similar tissue distribution to that of FPR1.While N-formyl peptides were the first peptides found to activate these receptors, the ligand diversity for FPR has proven to be quite broad and demonstrates to be both pro- and anti-inflammatory. They include peptidic ligands originating from bacterial and viral sources (including HIV), endogenous ligands such as chemokines and annexins, short peptides associated with inflammation and infection. Indeed, peptides from Herpes, Ebola and coronavirus 229E are ligands of FPR11.

Alternative names: FPR1, N-formyl peptide receptor 1, fMLP receptor

Gene ID: 2357

NCBI Accession: NM_002029

UniProt: P21462