FPR1 antibody (2nd Extracellular Loop)

Catalog No. ABIN7043135

The Rabbit Polyclonal anti-FPR1 antibody is suitable to detect FPR1 in samples from Human. It has been validated for WB, FACS and LCI.

Quick Overview for FPR1 antibody (2nd Extracellular Loop) (ABIN7043135)

Target: FPR1 (Formyl Peptide Receptor 1 (FPR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FPR1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-FPR1 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 179-192

Purpose: A Rabbit Polyclonal Antibody to Human FPR1

Predicted Reactivity: Human only Not recommended for use with rat,mouse samples

Characteristics: Anti-Human FPR1 (extracellular) Antibody (ABIN7043135, ABIN7044275 and ABIN7044276) is a highly specific antibody directed against an epitope of the human N-formyl peptide receptor 1. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize FPR1 from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)NFSPWTNDPKERIN, corresponding to amino acid residues 179-192 of human FPR1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for FPR1

Target: FPR1 (Formyl Peptide Receptor 1 (FPR1))

Alternative Name: FPR1

Background:

Synonyms: N-formyl peptide receptor 1, fMLP receptor

Description: Chemotactic factors from both Gram-positive and Gram-negative bacteria are short peptides with N-formyl methionine at the N-terminus (extensively reviewed in reference 1). These peptides are released from bacteria during infection and activate formyl peptide receptors (FPR), members of the G-protein coupled receptor (GPCR) superfamily. In humans, the FPR family consists mainly of three receptors, FPR1, FPR2/ALX (formerly FPRL1), and FPR3 (formerly FPRL2) which all couple to the Gi subtype of G-proteins and ultimately lead to the activation of phospholipase C and intracellular Ca2+ increase1,2.FPRL1, or FPR2/ALX as it is commonly called, is a seven transmembrane protein like all GPCRs. This receptor was originally cloned by screening a HL60 neutrophil cDNA library with a FPR1 cDNA probe3. FPR2/ALX shares 69 % identity with FPR1 and despite its high homology, it displays relatively low affinity for fmlf, the most potent N-formyl peptide released by bacteria3.FPR1 was originally found in neutrophils and later found to be distributed in myeloid and non-myeloid cells as is the case for FPR2/ALX and FPR3 (FPR3 though is not expressed in neutrophils). FPR1 is also expressed in multiple organs and tissues including epithelial cells in organs with secretory functions, endocrine cells, liver hepathocytes, smooth muscle cells and endothelial cells, brain spinal cord and both motor and sensory neurons4. FPR2/ALX has a similar tissue distribution to that of FPR1.While N-formyl peptides were the first peptides found to activate these receptors, the ligand diversity for FPR has proven to be quite broad and demonstrates to be both pro- and anti-inflammatory. They include peptidic ligands originating from bacterial and viral sources (including HIV), endogenous ligands such as chemokines and annexins, short peptides associated with inflammation and infection. Indeed, peptides from Herpes, Ebola and coronavirus 229E are ligands of FPR11.

Gene ID: 2357

NCBI Accession: NM_002029

UniProt: P21462