FFAR2 antibody (Extracellular)

Catalog No. ABIN7043139

This anti-FFAR2 antibody is a Rabbit Polyclonal antibody detecting FFAR2 in WB, FACS and LCI. Suitable for Human.

Quick Overview for FFAR2 antibody (Extracellular) (ABIN7043139)

Target: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FFAR2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-FFAR2 Antibody

Binding Specificity: AA 153-167, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)

Specificity: 2nd extracellular loop

Cross-Reactivity (Details): Not recommended for mouse or rat samples.

Predicted Reactivity: Human only

Characteristics: Anti-Human GPR43/FFAR2 (extracellular) Antibody (ABIN7043139, ABIN7044283 and ABIN7044284) is a highly specific antibody directed against an epitope of the human Free fatty acid receptor 2. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize FFAR2 from human samples only. It will not recognize the protein from rat or mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)TEQVRSGNEIT(S)YEN, corresponding to amino acid residues 153-167 of human FFAR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for FFAR2

Target: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Alternative Name: FFAR2

Background: G-protein coupled receptor 43, Free fatty acid receptor 2,Free fatty acids (FFAs) are essential nutrients that are also involved in signal transduction through FFA receptors, members of the G-protein coupled receptor superfamily.FFAR2/GPR43 is an FFA receptor that is activated specifically by short fatty acids (acetate, propionate and butyrate), that are the products of bacterial fermentation in the gut1.GPR43 is expressed in adipose tissue, intestines, and immune tissues2 and its stimulation by interaction with short fatty acids inhibits cAMP production, activates the extracellular signal-regulated kinase (ERK) cascade via interactions with the Gi/o family of G-proteins. This increases intracellular Ca2+ levels, and promotes activation of the mitogen-activated protein kinase (MAPK) cascade through interactions with the Gq family of G-proteins1.These processes, mediated by GPR43 receptor regulate the host energy homeostasis and hence GPR43 is shown to be related to metabolic disorders, such as obesity and diabetes1.Because of its pivotal role in energy homeostasis and in regulating many cellular processes, GPR43 has many phenotypic effects: mutant mice are hyper susceptible to developing intestinal carcinogenesis3, Klebsiella pneumoniae infection4 and to increased differentiation of bone marrow cells into osteoclasts5. Studies have shown the role of GPR43 in regulating inflammatory responses, which can lead to colitis and asthma6,7. The various activities mediated by GPR43 makes it an appealing therapeutic target.

Alternative names: GPR43/FFAR2, G-protein coupled receptor 43, Free fatty acid receptor 2

Gene ID: 2867

NCBI Accession: NM_005306

UniProt: O15552