FZD1 antibody (Extracellular, N-Term)

Catalog No. ABIN7886329

This Rabbit Polyclonal antibody specifically detects FZD1 in WB, FACS and LCI. It exhibits reactivity toward Rat.

Quick Overview for FZD1 antibody (Extracellular, N-Term) (ABIN7886329)

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-FZD1 Antibody

Binding Specificity: AA 238-250, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Frizzled-1

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Frizzled-1 (FZD1) (extracellular) Antibody (ABIN7043140, ABIN7044291 and ABIN7044292) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize FZD1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)PEFWTSNPQHGGG, corresponding to amino acid residues 238-250 of rat Frizzled-1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235337)

Blocking Peptide: (ABIN7235337)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for FZD1

Target: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Alternative Name: FZD1

Background:

Synonyms: Frizzled class receptor 1, Fz-1

Description: Frizzled class receptor 1 (also known as FZD1) belongs to the frizzled subfamily of G protein-coupled receptors. Currently, the frizzled family of GPCRs contains 10 frizzled proteins (FZD1-10) and Smoothened (SMO).Similar to other GPCRs, FZD1 consists of a single polypeptide with an extracellular N-terminus, an intracellular C-terminus, seven hydrophobic transmembrane domains (TM1-TM7) linked by three extracellular loops (ECL1-ECL3) and three intracellular loops (ICL1-ICL3). FZD1 is encoded by the firzzled-1 gene which is mainly expressed in the adult heart, placenta, lung, kidney, pancreas, prostate and ovary.FZD1 is activated by WNTs, which are cysteine-rich lipo-glycoproteins with fundamental functions in ontogeny and tissue homeostasis. Signaling through the WNT/FZD complex can lead to the activation of pertussis toxin-sensitive heterotrimeric G proteins, the elevation of intracellular calcium activation of cGMP-specific PDE6 and elevation of cAMP as well as RAC-1, JNK, Rho and Rho kinase signaling1.The relation between the FZD1 pathway and various pathologies has been researched extensively. Reduced levels of FZD1 can be found in the spinal cords of ALS transgenic mice, possibly due to the increase of FZD1 protein degradation or the inhibition of FZD1 protein expression in the spinal cord in the disease process of ALS transgenic mice2. The FZD1 signaling pathway has also been implicated in inflammatory bowel disease. Patients with ulcerative colitis exhibit reduced expression of FZD1. FZD1 is overexpressed in the multi drug resistant cancer cell subline MCF-7/ADM and seems to mediate multidrug resistance by regulating the Wnt/β-catenin pathway3.

Gene ID: 58868

NCBI Accession: NM_003505

UniProt: Q08463

Pathways: WNT Signaling, Asymmetric Protein Localization