FZD3 antibody (Intracellular)

Catalog No. ABIN7043142

Product Details anti-FZD3 Antibody

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Binding Specificity: AA 581-596, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal antibody to Frizzled-3 receptor.

Specificity: Intracellular, C-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - identical

Characteristics: A rabbit polyclonal antibody to Frizzled-3 receptor.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)KVSSYHGSLHRSRDGR, corresponding to amino acid residues 581 - 596 of mouse Frizzled-3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-FR023

Blocking Peptide: BLP-FR023

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for FZD3

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Alternative Name: FZD3 (FZD3 Products)

Background: Frizzled Class Receptor 3, Fz-3,Frizzled 3, encoded by FZD3 gene, is a receptor in the Wnt/β-catenin pathway, a conserved signaling pathway that regulates gene expression and controls diverse developmental processes 1. It belongs to the Frizzled protein family that contains multiple hydrophobic domains predicted to form seven transmembrane helices, similar to G protein coupled receptors. Conserved sequences are found in the Frizzled proteins extracellular cysteine-rich domain (CRD), the putative transmembrane domains, parts of the intracellular loops and a juxtamembrane motif, KTXXXW, found in the membrane-proximal C-terminal region of all Frizzleds. The C-terminal tails, however, are highly divergent 2. Frizzled-3 is constitutively expressed in murine melanocyte precursors, melanocytes and human melanocytes 3, it is also expressed in the epidermis of the developing embryo 4 and its expression levels are changing between different parts of the ovary throughout the process of embryogenesis 1. Because of its crucial role in regulating proper embryogenesis and its role in the development of the central nervous system, including synaptogenesis and structural plasticity, mutations in FZD3 gene were shown to have a significant association with the development of schizophrenia and other psychotic disorders 5.

Alternative names: Frizzled Class Receptor 3, Fz-3

Gene ID: 14365

NCBI Accession: NM_145866

UniProt: Q61086

Pathways: WNT Signaling, Tube Formation