G Protein-Coupled Receptor 132 antibody (Extracellular)

Catalog No. ABIN7043148

Product Details anti-G Protein-Coupled Receptor 132 Antibody

Target: G Protein-Coupled Receptor 132 (GPR132)

Binding Specificity: AA 181-195, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G Protein-Coupled Receptor 132 antibody is un-conjugated

Application: Western Blotting (WB), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to GPR132

Specificity: 2nd extracellular loop

Cross-Reactivity (Details): The antibody will not recognize rat or mouse samples.

Predicted Reactivity: Human only

Characteristics: Anti-Human GPR132/G2A (extracellular) Antibody (ABIN7043148, ABIN7044431 and ABIN7044432) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPR132 from human samples only. The antibody will not recognize GPR132 from mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EDKET(S)FDMLQMDSR, corresponding to amino acid residues 181 - 195 of human GPR132

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for G Protein-Coupled Receptor 132

Target: G Protein-Coupled Receptor 132 (GPR132)

Alternative Name: GPR132 (GPR132 Products)

Background: G protein-coupled receptor 132, G2 accumulation protein, G2A,G-protein coupled receptor 132, (GPR132, G2A) is classified as a member of the proton sensing G-protein coupled receptor (GPCR) subfamily. Like other members of this subfamily, i.e. GPR4, OGR1 (GPR68), and TDAG8 (GPR65), G2A is expressed on the cell surface membrane and contains seven transmembrane domains, similar to serpentine receptors. GPR132 senses changes in extracellular pH , and activation of this proton-sensing receptor by acidosis transduces multiple downstream G protein signaling pathways1.GPR132 is expressed in a broad range of immunoregulatory cell types, including macrophages, dendritic cells, neutrophils, mast cells, T and B lymphocytes. It mediates immune cell migration as well as phagocytosis and regulates autoimmunity and lymphocyte homeostasis2.G2A also plays a role in tumor suppression: the tumor microenvironment is acidic due to glycolytic cancer cell metabolism, hypoxia, and deficient blood perfusion and hence, G2A and other proton sensing receptors may be involved in regulation of cancer cell metastasis and proliferation. Small molecule modulators of the pH -sensing GPCRs are being actively developed and evaluated. Research on pH -sensing GPCRs provides important insights into the molecular interaction between the tumor and its acidic microenvironment and may identify new targets for cancer therapy3.

Alternative names: G protein-coupled receptor 132, G2 accumulation protein, G2A

Gene ID: 29933

NCBI Accession: NM_013345

UniProt: Q9UNW8