GPR17 antibody (Intracellular)

Catalog No. ABIN7043149

Product Details anti-GPR17 Antibody

Target: GPR17 (G Protein-Coupled Receptor 17 (GPR17))

Binding Specificity: AA 220-232, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR17 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to GPR17 Receptor

Specificity: Intracellular, 3rd loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Mouse - identical

Characteristics: Anti-GPR17 Antibody (ABIN7043149, ABIN7044435 and ABIN7044436)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR17 from rat and mouse samples. It is unlikely to recognize the protein from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RQGPRIEKHLKNK, corresponding to amino acid residues 220-232 of rat GPR17

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GR048

Blocking Peptide: BLP-GR048

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR17

Target: GPR17 (G Protein-Coupled Receptor 17 (GPR17))

Alternative Name: GPR17 (GPR17 Products)

Background: G-protein coupled receptor 17, Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT Receptor, P2Y-like receptor,GPR17 is an orphan receptor that can be double-activated by uracil and cysteinyl-leukotriene (CysLT). It is phylogenetically related to both purinergic P2Y and CysLT receptors. GPR17 belongs to the superfamily of G-protein coupled receptors (GPCRs)4.GPR17 expression is highly detected in the brain, kidney, and heart. In the brain, GPR17 is mainly distributed in white matter, gray matter, subependymal zone, and corpus callosum1.Studies have shown that GPR17 receptor plays an important role in spinal cord injury, myelin sheath injury, cerebral ischemic injury, where its expression is extremely upregulated1,2.GPR17 activation promotes oligodendrocyte differentiation and progression toward mature myelin-forming cells. Thus, GPR17 modulators may be used as therapeutic tools to treat pathologies associated with myelin repair in CNS demyelinating diseases3.

Alternative names: GPR17, G-protein coupled receptor 17, Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT Receptor, P2Y-like receptor

Gene ID: 767613

NCBI Accession: NM_001161415

UniProt: Q09QM4