GPR17 antibody (Intracellular, Loop 3)

Catalog No. ABIN7886434

This Rabbit Polyclonal antibody specifically detects GPR17 in WB, IF and IHC. It exhibits reactivity toward Rat.

Quick Overview for GPR17 antibody (Intracellular, Loop 3) (ABIN7886434)

Target: GPR17 (G Protein-Coupled Receptor 17 (GPR17))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR17 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-GPR17 Antibody

Binding Specificity: AA 220-232, Intracellular, Loop 3

Purpose: A Rabbit Polyclonal Antibody to GPR17 Receptor

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Mouse - identical

Characteristics: Anti-GPR17 Antibody (ABIN7043149, ABIN7044435 and ABIN7044436) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR17 from rat and mouse samples. It is unlikely to recognize the protein from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RQGPRIEKHLKNK, corresponding to amino acid residues 220-232 of rat GPR17

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235376)

Blocking Peptide: (ABIN7235376)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GPR17

Target: GPR17 (G Protein-Coupled Receptor 17 (GPR17))

Alternative Name: GPR17

Background:

Synonyms: G-protein coupled receptor 17, Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT Receptor, P2Y-like receptor

Description: GPR17 is an orphan receptor that can be double-activated by uracil and cysteinyl-leukotriene (CysLT). It is phylogenetically related to both purinergic P2Y and CysLT receptors. GPR17 belongs to the superfamily of G-protein coupled receptors (GPCRs)4.GPR17 expression is highly detected in the brain, kidney, and heart. In the brain, GPR17 is mainly distributed in white matter, gray matter, subependymal zone, and corpus callosum1.Studies have shown that GPR17 receptor plays an important role in spinal cord injury, myelin sheath injury, cerebral ischemic injury, where its expression is extremely upregulated1,2.GPR17 activation promotes oligodendrocyte differentiation and progression toward mature myelin-forming cells. Thus, GPR17 modulators may be used as therapeutic tools to treat pathologies associated with myelin repair in CNS demyelinating diseases3.

Gene ID: 767613

NCBI Accession: NM_001161415

UniProt: Q09QM4