GPR183 antibody (Extracellular)

Catalog No. ABIN7043152

Product Details anti-GPR183 Antibody

Target: GPR183 (G Protein-Coupled Receptor 183 (GPR183))

Binding Specificity: AA 18-31, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR183 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal antibody to GPR183 (extracellular).

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - 13 out of 14 amino acid residues identical Mouse,rat - 12 out of 14 amino acid residues identical

Characteristics: Anti-GPR183 (extracellular) Antibody (ABIN7043152, ABIN7044457 and ABIN7044458)) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR183 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GND(S)DLYAHHSTAR, corresponding to amino acid residues 18 - 31 of human GPR183

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GR063

Blocking Peptide: BLP-GR063

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR183

Target: GPR183 (G Protein-Coupled Receptor 183 (GPR183))

Alternative Name: GPR183 (GPR183 Products)

Background: G-Protein Coupled Receptor 183, Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2, EBI2,G-protein coupled receptor 183 (GPR183) also known as Epstein-Barr virus-induced gene 2 (EBI2), is a chemotactic receptor that participates in the migratory capability of cells 2. It belongs to the rhodopsin-like subfamily of class A transmembrane spanning (7TM) G protein-coupled receptors and its most potent ligand is oxysterol 7α-25-OHC 1.Dihydroxycholesterols, such as oxysterol 7α-25-OHC, are generated from cholesterol in steady state but also in the context of inflammation. GPR183 senses these oxysterols and induces chemotactic migration of immune cells towards higher concentrations of these ligands and hence cells that express GPR183 are trafficking through an oxysterol gradient dependent manner acting like chemokine processes. The migratory function of this receptor affects several important immune processes, in particular, GPR183 is involved in the T-dependent antibody response in the germinal centers. This puts GPR183 in a unique position to effect autoimmunity and inflammatory bowel diseases 3,4.In addition, GPR183 is highly expressed by type 3 innate lymphoid cells (ILC3) and its oxysterol ligand was shown to be essential for the localization and the migration of ILC3s and to have a critical role for the formation of lymphoid tissues in the mouse colon during colitis 2.

Alternative names: G-Protein Coupled Receptor 183, Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2, EBI2

Gene ID: 1880

NCBI Accession: NM_004951

UniProt: P32249