GPR34 antibody (Extracellular) (FITC)

Catalog No. ABIN7043154

Product Details anti-GPR34 Antibody (FITC)

Target: GPR34 (G Protein-Coupled Receptor 34 (GPR34))

Binding Specificity: AA 197-209, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR34 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal Antibody to GPR34 Conjugated to the Fluorescent Dye FITC

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - 12,13 amino acid residues identical

Characteristics: Anti-GPR34 (extracellular) Antibody (ABIN7043153, ABIN7044449 and ABIN7044450)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize GPR34 from human, mouse, and rat samples. \nAnti-GPR34 (extracellular)-FITC Antibody (ABIN7043153, ABIN7044449 and ABIN7044450)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide CFHYRDRHNAKGE, corresponding to amino acid residues 197 - 209 of mouse GPR34

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: RIC-001-F

Blocking Peptide: BLP-GR055

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR34

Target: GPR34 (G Protein-Coupled Receptor 34 (GPR34))

Alternative Name: GPR34 (GPR34 Products)

Background: G-protein coupled receptor 34,The G-protein-coupled receptor 34 (GPR34) is currently classified as an orphan receptor of the P2Y12-like receptor group. The receptor belongs to the super family of G-protein coupled receptors (GPCRs)1.The N-terminus of human GPR34 has three putative N-glycosylation sites (NXS/T), and the C-terminus has a cysteine residue which is palmitoylated in many GPCRs. GPR34 contains several putative phosphorylation sites primed by a serine or threonine in the C-terminus2.GPR34 is highly expressed in immune cells such as mast cells, macrophages, microglia, and immune system-derived cell lines (HL-60, K562, WEHI-3B, RAW 264.7, P815). Studies show that GPR34 function is required to arrest microglia in the M0 homeostatic non-phagocytic phenotype and downregulation of GPR34 promotes the neurodegenerative microglia phenotype2,3,4.GPR34 is linked to several human diseases including immunological diseases and cancer. Most known effects of GPR34 are immune function-related. For example, GPR34 is significantly down-regulated in Mycobacterium tuberculosis-infected macrophages, GPR34 mRNA is significantly lower in the cerebrospinal fluid from multiple sclerosis patients, GPR34 is significantly upregulated in the striatum and cortex of patients with Parkinson's disease but downregulated in substantia nigra. elevated GPR34 also plays a role in the progression of some tumors, such as human gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and colorectal cancer1-3.

Alternative names: G-protein coupled receptor 34

Gene ID: 23890

NCBI Accession: NM_005300

UniProt: Q9R1K6