GPR35 antibody (Extracellular)

Catalog No. ABIN7043156

The Rabbit Polyclonal anti-GPR35 antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect GPR35 in samples from Mouse.

Quick Overview for GPR35 antibody (Extracellular) (ABIN7043156)

Target: GPR35 (G Protein-Coupled Receptor 35 (GPR35))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR35 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR35 Antibody

Binding Specificity: AA 156-170, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GPR35 (extracellular)

Specificity: Extracellular, 2nd loop.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Mouse - 14 out of 15 amino acid residues identical, rat - 12 out of 15 amino acid residues identical Not recommended for human samples

Characteristics: Anti-GPR35 (extracellular) Antibody (ABIN7043156, ABIN7044441 and ABIN7044442) is a highly specific antibody directed against an extracellular epitope of the mouse GPR35. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR35 from mouse and rat samples. The antibody will not recognize the receptor from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EGGF(S)FSSQTRRNFS, corresponding to amino acid residues 156 - 170 of mouse GPR35

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235400)

Blocking Peptide: (ABIN7235400)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR35

Target: GPR35 (G Protein-Coupled Receptor 35 (GPR35))

Alternative Name: GPR35

Background: G-Protein Coupled Receptor 35, Kynurenic Acid Receptor,G- protein coupled receptor 35 (GPR35) is an "orphan" GPCR, whose native ligand is still unknown. It was shown to be involved in the modulation of synaptic transmission, nociception, neuropathic and inflammatory pain, and in various diseases including ulcerative colitis and primary sclerosing cholangitis1 and hence there is a great therapeutic potential in studying its activity.In vitro, GPR35 is stimulated by high concentrations of a number of small molecules, including kynurenic acid, 2-oleoyllysophosphatidic acid, DHICA, reverse T3, cGMP and it has a wide range of synthetic agonists and antagonists that either activate or inactivate it, respectively (Summarized in2). GPR35 is composed of seven trans-membrane domains, and it was shown to share structural homology with various receptors for chemokines, chemotactic cytokines that direct the traffic of leukocytes and other cells in the body. It was shown to be activated by chemokine CXCL17, and hence the role of a chemokine receptor was also assigned to it3.GPR35 expression has been identified within discrete regions of the central nervous system (CNS) and peripheral nervous system, and lower levels in heart, lung and skeletal muscle, and also in the lower gut and colon and by various white cell groups, including numerous dendritic cell and monocyte populations4.

Alternative names: G-Protein Coupled Receptor 35, Kynurenic Acid Receptor

Gene ID: 64095

NCBI Accession: NM_001195382

UniProt: Q9ES90