GPR4 antibody (2nd Extracellular Loop)

Catalog No. ABIN7886428

The Rabbit Polyclonal anti-GPR4 antibody (ABIN7886428) specifically detects GPR4 in WB, IHC, FACS and LCI. The antibody is reactive with Rat samples.

Quick Overview for GPR4 antibody (2nd Extracellular Loop) (ABIN7886428)

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR4 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 162-177

Purpose: A Rabbit Polyclonal Antibody to GPR4

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,human - 15,16 amino acid residues identical

Characteristics: Anti-GPR4 (extracellular) Antibody (ABIN7043160, ABIN7044423 and ABIN7044424) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GPR4 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235415)

Blocking Peptide: (ABIN7235415)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GPR4

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Alternative Name: GPR4

Background:

Synonyms: Acid-sensing G-protein coupled receptor 4, GPR19

Description: G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.

Gene ID: 308408

NCBI Accession: NM_005282

UniProt: Q4KLH9