GPR55 antibody (Extracellular)

Catalog No. ABIN7043162

Product Details anti-GPR55 Antibody

Target: GPR55 (G Protein-Coupled Receptor 55 (GPR55))

Binding Specificity: AA 261-275, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR55 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to GPR55

Specificity: 3rd extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: rat - 11,15 amino acid residues identical,Mouse - 14

Characteristics: Anti-GPR55 (extracellular) Antibody (ABIN7043162, ABIN7044202 and ABIN7044203)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human, rat, and mouse samples. The antibody will not recognize the human protein.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RNRFILD(S)RMKQGIS, corresponding to amino acid residues 261 - 275 of mouse GPR55

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: BLP-CR062

Blocking Peptide: BLP-CR062

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR55

Target: GPR55 (G Protein-Coupled Receptor 55 (GPR55))

Alternative Name: GPR55 (GPR55 Products)

Background: G-Protein Coupled Receptor 55,G protein coupled receptor 55 (GPR55) is a cannabinoid receptor activated by a number of endo-, phyto- and synthetic cannabinoid ligands such as THC, JWH015 and anandamide1-3.GPR55 is only 13.5 % identical to the canonic cannabinoid receptor CB1 and 14.4 % identical to CB2. Upon ligand binding, it initiates a signaling cascade quite distinct from those used by CB1 and CB2 that eventually leads to an increase in intracellular calcium levels. GPR55 belongs to the Class A GPCRs. It shares many similarities with rhodopsin and as all other G-protein coupled receptors in its basic structure of seven transmembrane domains, intracellular C- terminus and extracellular N-terminal extensions4. The protein is highly expressed in large dorsal root ganglion neurons and its mRNA is present in the brain and periphery1.GPR55 has a role in self-renewing adult neural stem cell development and in hippocampal neurogenesis3. Experiments with mice in which GPR55 has been inactivated reveal a role for this receptor in neuropathic and inflammatory pain as well as in bone physiology. Thus deciphering the pharmacology of this receptor could lead to new therapeutics in these fields5.

Alternative names: G-Protein Coupled Receptor 55

Gene ID: 227326

NCBI Accession: NM_005683

UniProt: Q3UJF0