Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (AA 86-109), (Extracellular) antibody

Catalog No. ABIN7043165

The Rabbit Polyclonal anti- antibody has been validated for FACS, WB and LCI. It is suitable to detect in samples from Rat.

Quick Overview for Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (AA 86-109), (Extracellular) antibody (ABIN7043165)

Target: Adhesion G Protein-Coupled Receptor G1 (ADGRG1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Live Cell Imaging (LCI)

Product Details

Binding Specificity: AA 86-109, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GPR56

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): This antibody recognizes the N-terminal fragment (GPR56-NT), that can be secreted to the media.

Predicted Reactivity: 14 amino acid residues identical,Mouse - 13, human - 11

Characteristics: Anti-GPR56 (extracellular) Antibody (ABIN7043165, ABIN7044433 and ABIN7044434) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect flow cytometry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize ADGRG1 from rat, mouse, and human samples. The antibody recognizes the N-terminal fragment (GPR56-NT) that can be secreted to the media.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RHTGRLHLRYGKND, corresponding to amino acid residues 86-109 of rat GPR56

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235431)

Blocking Peptide: (ABIN7235431)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details

Target: Adhesion G Protein-Coupled Receptor G1 (ADGRG1)

Alternative Name: ADGRG1

Background: G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1,GPR56 (ADGRG1) is a member of the adhesion G-protein coupled receptor (GPCR) family, responsible for the regulation of cell adhesion, migration, polarity, and guidance. GPR56 levels are increased in many cancer types and seems to be relevant for tumor cell migration and invasion1,2. GPR56 structure is characterized by a long extracellular domain that is involved in cell-to-cell and cell-to-extracellular matrix interactions. The receptor contains a GPS motif, responsible for cleaving the protein. Seven different glycosylation sites along its N-terminal fragment are present1-3.Expression of Gpr56 mRNA can be found in neuronal progenitor cells of the cerebral cortical ventricular and subventricular zones during periods of neurogenesis. Studies show that GPR56 protein knockdown promotes mesenchymal differentiation of glioma stem-like initiating cells, accompanied by increased radioresistance in vitro and in vivo1-3.Mutations in GPR56 may cause bilateral frontoparietal polymicrogyria, a cobblestone-like cortical malformation, characterized by overmigrating neurons and the formation of neuronal ectopias on the surface of the brain2,3.

Alternative names: GPR56, G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1

Gene ID: 260326

NCBI Accession: NM_001145770

UniProt: Q8K3V3