GPR68 antibody (Extracellular)

Catalog No. ABIN7043170

Product Details anti-GPR68 Antibody

Target: GPR68 (G Protein-Coupled Receptor 68 (GPR68))

Binding Specificity: AA 169-183, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR68 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to GPR68 (OGR1)

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,human - 14,15 amino acid residues identical

Characteristics: Anti-GPR68 (OGR1) (extracellular) Antibody (ABIN7043170, ABIN7044425 and ABIN7044426)) is a highly specific antibody directed against an epitope of the mouse ovarian cancer G-protein coupled receptor 1. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR68 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IFC

Negative Control: BLP-GR042

Blocking Peptide: BLP-GR042

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR68

Target: GPR68 (G Protein-Coupled Receptor 68 (GPR68))

Alternative Name: GPR68 (GPR68 Products)

Background: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor,All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54 % ) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.

Alternative names: GPR68/OGR1, Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Gene ID: 238377

NCBI Accession: NM_001177676

UniProt: Q8BFQ3