GPR77 antibody (Extracellular)

Catalog No. ABIN7043172

Product Details anti-GPR77 Antibody

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Binding Specificity: AA 4-18, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR77 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor 2

Specificity: Extracellular, N-terminus

Cross-Reactivity (Details): Will not recognize C5a Anaphylatoxin Chemotactic Receptor 2 from rat or mouse samples.

Predicted Reactivity: Human only

Characteristics: Anti-Human C5aR2 (extracellular) Antibody (ABIN7043172, ABIN7043928 and ABIN7043929) is a highly specific antibody directed against an epitope of human C5a anaphylatoxin receptor 2 (C5aR2, C5L2). The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is likely to detect the receptor in living cells. It has been designed to recognize C5L2 from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DSVSYEYGDYSDLSD, corresponding to amino acid residues 4-18 of human C5a anaphylatoxin chemotactic receptor 2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR77

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Alternative Name: C5AR2 (GPR77 Products)

Background: C5a anaphylatoxin chemotactic receptor 2, C5a anaphylatoxin receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5L2, GPR77,Complement fragment C5a is a pro-inflammatory polypeptide produced during activation of the complement cascade of serum proteins in response to foreign surfaces such as microorganisms and tissue damaged by physical or chemical injury. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77).Expression of C5L2 (C5a Anaphylatoxin Receptor 2) can be found in various tissues and cells such as astrocytes, neutrophils/macrophages, mast cells, immature dendritic cells, as well as in the brain, lung, heart, kidney, liver, ovary or testis.The C5L2 receptor is a G-protein coupled receptor clustered with other chemoattractant receptors, such as type-II angiotensin-II receptor, bradykinin receptors, the formyl peptide receptor family, ChemR23 and several orphan GPCRs. A small number of single-nucleotide polymorphisms (SNP) have been found in the receptor gene. C5L2 has two synonymous SNPs at 614G/A and 860C, however there are no known associations between these SNPs and human disease.Although C5L2 has the conventional structure of a GPCR, studies have shown that C5L2 does not couple to G proteins owing to the lack of a highly conserved Asp-Arg-Tyr motif, found in the third TM domain1.

Alternative names: C5aR2, C5a anaphylatoxin chemotactic receptor 2, C5a anaphylatoxin receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5L2, GPR77

Gene ID: 27202

NCBI Accession: NM_018485

UniProt: Q9P296