GPR83 antibody (Extracellular)

Catalog No. ABIN7043174

The Rabbit Polyclonal anti-GPR83 antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect GPR83 in samples from Mouse.

Quick Overview for GPR83 antibody (Extracellular) (ABIN7043174)

Target: GPR83 (G Protein-Coupled Receptor 83 (GPR83))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR83 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR83 Antibody

Binding Specificity: AA 59-71, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GPR83

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - identical

Characteristics: Anti-GPR83 (extracellular) Antibody (ABIN7043174, ABIN7044445 and ABIN7044446) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPR83 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RRYGAESQNPTVK, corresponding to amino acid residues 59 - 71 of mouse GPR83

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:3000

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235450)

Blocking Peptide: (ABIN7235450)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR83

Target: GPR83 (G Protein-Coupled Receptor 83 (GPR83))

Alternative Name: GPR83

Background: G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR,GPR83 (JP05, GIR, GPR72) is a G-protein coupled receptor (GPCR) highly expressed in the brain, spleen and thymus, suggesting that this receptor may be targeted to treat neurological and immune disorders1.GPCR is a family of receptors responsible for initiation of a myriad of intracellular signaling cascades, these receptors are characterized by an extracellular N-terminus, intracellular C-terminal tail, and seven membrane-spanning α-helices (TM-1 to TM-7)2.In the brain GPR83 is expressed in the hippocampus, amygdala, prefrontal cortex, and in various hypothalamic nuclei where it may play a significant role in learning memory, reward, emotional behaviors, and stress regulation3. GPR83 is also expressed in regulatory T cells (Tregs) and it could potentially be used as a specific marker used to identify natural Tregs4.GPR83 was considered as an "orphan" GPCR. Recently, PEN, a neuropeptide was identified as its endogenous ligand1.

Alternative names: G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR

Gene ID: 14608

NCBI Accession: NM_016540

UniProt: P30731