GPR84 antibody (2nd Extracellular Loop) (FITC)

Catalog No. ABIN7043176

This Rabbit Polyclonal antibody specifically detects GPR84 in FACS and LCI. It exhibits reactivity toward Human.

Quick Overview for GPR84 antibody (2nd Extracellular Loop) (FITC) (ABIN7043176)

Target: GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR84 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR84 Antibody (FITC)

Binding Specificity: 2nd Extracellular Loop, AA 166-180

Purpose: A Rabbit Polyclonal Antibody to GPR84 Conjugated to the Fluorescent Dye FITC

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - 14,15 amino acid residues identical, mouse,rat - 13

Characteristics: Anti-GPR84 (extracellular) Antibody (ABIN7043175, ABIN7044443 and ABIN7044444) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR84 from human, rat, and mouse samples. \nAnti-GPR84 (extracellular)-FITC Antibody (ABIN7043175, ABIN7044443 and ABIN7044444)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogen: CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84

Isotype: IgG

Application Details

Application Notes:

WB: The optimal concentration should be determined by the user

FC: 2.5-5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235454)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GPR84

Target: GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Alternative Name: GPR84

Background:

Synonyms: G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

Description: GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive Gi/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer1-3.GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production1-3.

Gene ID: 53831

NCBI Accession: NM_020370

UniProt: Q9NQS5