GABARAP antibody (Intracellular)

Catalog No. ABIN7043179

This Rabbit Polyclonal antibody specifically detects GABARAP in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for GABARAP antibody (Intracellular) (ABIN7043179)

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABARAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GABARAP Antibody

Binding Specificity: AA 2-15, Intracellular

Purpose: A Rabbit Polyclonal Antibody to GABA Receptor Associated Protein

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Due to high homology with GABA receptor associated protein-like proteins the antibody will recognize:GABA receptor associated protein-like 1 (11/14 amino acid residues identical), (Accession Q0VGK0).GABA receptor associated protein-like 3 (11/14 amino acid residues identical), (Accession Q9BY60).May recognize GABA receptor associated protein-like 2 (8/14 amino acid residues identical), (Accession P60522).

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-GABARAP Antibody (ABIN7043179, ABIN7044532 and ABIN7044533) is a highly specific antibody directed against an extracellular epitope of the rat GABA receptor associated protein. The antibody can be used in western blot analysis. It has been designed to recognize GABARAP from human, mouse and rat samples. Due to high homology with GABA receptor associated protein-like proteins the antibody will recognize GABA receptor associated protein 1 and 3 and may recognize GABA receptor associated protein 2.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KFVYKEEHPFEKRR, corresponding to amino acid residues 2-15 of rat GABARAP

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:800

Comment:

Negative Control: (ABIN7235469)

Blocking Peptide: (ABIN7235469)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GABARAP

Target: GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

Alternative Name: GABARAP

Background: GABA receptor associated protein, GABARAPL,GABA(A) receptors are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABA associated protein (GABARAP) family interacts specifically with the γ1, γ2S and γ2L subunits of the GABA(A) receptor. GABARAP is expressed in a variety of tissues including heart, brain, placenta, liver and others. The genetic sequence encoding GABARAP is similar to microtubule-associated proteins 1A and 1B1.GABARAP has an asymmetric structure with a basic N-terminal region and an acidic C terminus. GABARAP has a compact fold consisting of four or five-stranded β-sheets with two α-helices on each side. The outer strands of the β-sheets are aligned anti-parallel to the inner strands and helices α3 and α4 are located on one side of the sheet. The presence of α1 and α2 is a unique feature of GABARAP and forms a hydrophilic patch suitable for protein-protein interface. GABARAP exists in a monomeric conformation at low protein concentration and a head-to-tail oligomeric conformation in high salt conditions.GABARAP is involved in intracellular trafficking and is an integral part of the intra-Golgi transport machinery. Thus it is detected in cellular compartments such as the Golgi apparatus and endoplasmic reticulum. GABARAP affects GABA(A) receptors' cell surface expression by increasing their intracellular trafficking. Disruption of cellular microtubules prevents the clustering of both GABARAP and GABA(A) receptors. GABARAP also interacts with other receptors such as the glycine receptor at the postsynaptic site2.

Alternative names: GABARAP, GABA receptor associated protein, GABARAPL

Gene ID: 58974

NCBI Accession: NM_007278

UniProt: P60517

Pathways: Autophagy