GALR3 antibody (Extracellular)

Catalog No. ABIN7043182

Product Details anti-GALR3 Antibody

Target: GALR3 (Galanin Receptor 3 (GALR3))

Binding Specificity: AA 159-172, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALR3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Galanin Receptor Type 3

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-GALR3 (extracellular) Antibody (ABIN7043182, ABIN7044400 and ABIN7044401)) is a highly specific antibody directed against an epitope of the mouse Galanin receptor 3. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GALR3 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: SYYGTVRYGALELC, corresponding to amino acid residues 159-172 of mouse Galanin receptor type 3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GR013

Blocking Peptide: BLP-GR013

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GALR3

Target: GALR3 (Galanin Receptor 3 (GALR3))

Alternative Name: GALR3 (GALR3 Products)

Background: Galanin receptor type 3, GALNR3, GAL3R,Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.1-4Based on mRNA, the GALR1 is predominantly distributed in the central nervous system, spinal cord and dorsal root ganglia while GALR2 is abundant in both central and peripheral nervous systems. GALR3 mRNA is distributed in peripheral tissues such as the heart, spleen, and testis. However, its expression in the brain is still controversial.4,5

Alternative names: GALR3, Galanin receptor type 3, GALNR3, GAL3R

Gene ID: 14429

NCBI Accession: NM_003614

UniProt: O88853

Pathways: cAMP Metabolic Process, Feeding Behaviour