GABRQ antibody (Extracellular)

Catalog No. ABIN7043197

Product Details anti-GABRQ Antibody

Target: GABRQ (gamma-aminobutyric Acid (GABA) Receptor, theta (GABRQ))

Binding Specificity: AA 131-143, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRQ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to GABA(A) θ Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-GABA(A) teta Receptor (GABRQ) (extracellular) Antibody is directed against an extracellular epitope of mouse γ-aminobutyric acid receptor subunit θ. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (ABIN7043197, ABIN7044315 and ABIN7044316) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize GABRQ from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: DYRMHEKLWVPDC, corresponding to amino acid residues 131-143 of mouse GABRQ

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GABRQ

Target: GABRQ (gamma-aminobutyric Acid (GABA) Receptor, theta (GABRQ))

Alternative Name: GABRQ (GABRQ Products)

Background: γ-Aminobutyric acid receptor subunit θ, GABA(A) receptor subunit theta,The A-type receptors for γ-aminobutyric acid (GABA(A)) mediate the majority of fast inhibitory transmission in the mammalian central nervous system. Many clinically important drugs including benzodiazepines, barbiturates and general anesthetics act as positive allosteric modulators of this receptor1. GABA(A) receptors are pentameric ligand-gated ion-channels, composed of subunits from many subunit classes: α1-α6, β1-β4, γ1-γ4, δ, ε , θ, π and ?1-?32. The arrangement of subunits around the channel is probably γβαβα counter-clockwise when viewed from the extracellular space, ε and π subunits can replace the γ and δ subunit within the pentamer, whereas the θ subunit could replace a β subunit3.The localized expression of this subunit is consistent with the fact that all GABA(A) receptor subunit genes have distinct expression patterns in the brain3. The θ subunit has been reported to be strongly expressed in the striatum and has been localized to the locus ceruleus4.Impairment of the γ-aminobutyric acid (GABA) signaling system is believed to partially account for behavioral and cognitive deficits associated with schizophrenia and mood disorders5. Reduction of GABA(A) mediated signal transmission has also been associated with anxiety, panic, impaired learning and memory6.

Alternative names: GABA(A) theta Receptor (GABRQ), gamma-Aminobutyric acid receptor subunit theta, GABA(A) receptor subunit theta

Gene ID: 57249

NCBI Accession: NM_018558

UniProt: Q9JLF1